PMID: 2094420Jan 1, 1990Paper

Multicomponent spectrophotometric assay of riboflavine and photoproducts

Journal of Pharmaceutical and Biomedical Analysis
I Ahmad, H D Rapson

Abstract

A multicomponent spectrophotometric method has been developed for the simultaneous determination of riboflavine, formylmethylflavine and degradation products in photolysed solutions. It is based on partial separation of the photoproducts by chloroform extraction at pH 2.0 in a potassium chloride-hydrochloric acid solution and subsequent determination, in the aqueous phase, of riboflavine and formylmethylflavine at 445 and 385 nm. The chloroform extract containing lumichrome and lumiflavine is evaporated to dryness, the residue dissolved in acetate buffer (pH 4.5) and the products determined at 356 and 445 nm. The reproducibility of the method, based on the analysis of synthetic mixtures, is within +/- 5%. Absorption corrections for minor products and interfering substances have been proposed. Chromatographic, spectrophotometric and distribution coefficient data for riboflavine and photoproducts are reported. The method is specific, rapid and convenient for photodegradation studies of riboflavine and formylmethylflavine.

References

Apr 1, 1977·Photochemistry and Photobiology·W W Moore, R C Ireton
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Citations

Jul 6, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Iqbal AhmadFaiyaz H M Vaid
Dec 3, 2009·Chemical & Pharmaceutical Bulletin·Iqbal AhmadFaiyaz Hussain Madni Vaid
Jul 13, 2004·Journal of Photochemistry and Photobiology. B, Biology·Iqbal AhmadFaiyaz H M Vaid
Jul 22, 2004·International Journal of Pharmaceutics·Iqbal AhmadQ Nawab Manzar Ali
Jun 10, 2017·Journal of Photochemistry and Photobiology. B, Biology·Iqbal AhmadSaif-Ur-Rehman Khattak
May 2, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Eduard StadlerGeorg Gescheidt
Sep 28, 2018·Luminescence : the Journal of Biological and Chemical Luminescence·Tania MirzaIqbal Ahmad
Sep 24, 2014·Beilstein Journal of Organic Chemistry·Muhammad Ali SherazIqbal Ahmad
Feb 24, 2015·AAPS PharmSciTech·Iqbal AhmadAmbreen Hafeez
Jul 10, 2018·Luminescence : the Journal of Biological and Chemical Luminescence·Iqbal AhmadSaif-Ur-Rehman Khattak
Jan 14, 2020·Journal of Photochemistry and Photobiology. B, Biology·Zubair AnwarIqbal Ahmad
Jan 1, 1992·Journal of Pharmaceutical and Biomedical Analysis·I AhmadA A Fareedi

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