Multicopper manganese oxidase accessory proteins bind Cu and heme

Biochimica Et Biophysica Acta
Cristina N ButterfieldBradley M Tebo

Abstract

Multicopper oxidases (MCOs) catalyze the oxidation of a diverse group of metal ions and organic substrates by successive single-electron transfers to O2 via four bound Cu ions. MnxG, which catalyzes MnO2 mineralization by oxidizing both Mn(II) and Mn(III), is unique among multicopper oxidases in that it carries out two energetically distinct electron transfers and is tightly bound to accessory proteins. There are two of these, MnxE and MnxF, both approximately 12kDa. Although their sequences are similar to those found in the genomes of several Mn-oxidizing Bacillus species, they are dissimilar to those of proteins with known function. Here, MnxE and MnxF are co-expressed independent of MnxG and are found to oligomerize into a higher order stoichiometry, likely a hexamer. They bind copper and heme, which have been characterized by electron paramagnetic resonance (EPR), X-ray absorption spectroscopy (XAS), and UV-visible (UV-vis) spectrophotometry. Cu is found in two distinct type 2 (T2) copper centers, one of which appears to be novel; heme is bound as a low-spin species, implying coordination by two axial ligands. MnxE and MnxF do not oxidize Mn in the absence of MnxG and are the first accessory proteins to be required by an MC...Continue Reading

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Citations

Jan 28, 2017·Metallomics : Integrated Biometal Science·Cristina N Butterfield, Bradley M Tebo
Feb 2, 2018·Journal of the American Society for Mass Spectrometry·Mowei ZhouLjiljana Paša-Tolić
Jul 20, 2017·Chemistry : a European Journal·Alexandr N SimonovLeone Spiccia
Oct 1, 2017·Nature Communications·Christine A RomanoBradley M Tebo
Jun 21, 2020·Topics in Current Chemistry·Daily Rodriguez-PadronAlain R Puente Santiago
Jul 4, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lizhi TaoR David Britt
Jul 18, 2017·Journal of the American Chemical Society·Alexandra V SoldatovaThomas G Spiro
Jul 18, 2017·Journal of the American Chemical Society·Alexandra V SoldatovaThomas G Spiro

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