Multidepth screening of living cells using optical waveguides

Biosensors & Bioelectronics
Robert HorvathJeremy J Ramsden

Abstract

The use of planar optical waveguides as substrata for label-free, non-invasive monitoring of cells growing on them is demonstrated. Different submicrometre depths (measured from and perpendicular to the substratum surface) can be selected for monitoring. The so-called symmetry waveguide configuration with a low refractive index waveguide support (nanoporous silica with refractive index approximately 1.2) and a polystyrene waveguiding film with a heat-embossed grating coupler is exploited to obtain practically useful differences between the penetration depths of different waveguide modes. Robust data processing techniques are developed to obtain quantitative information about the cell refractive index profile perpendicular to the substratum from the measured effective refractive indices of the modes. In particular, a method is introduced with which cell refractive index variations above and below a predefined and tunable depth can be separated using two modes. The technique can be extended to more modes to gain even more comprehensive information from predefined submicrometre slices of the cell layer. The introduced methods are also suitable for monitoring the kinetics of changes in cell refractive index profiles.

Citations

Jun 5, 2012·Biomicrofluidics·Nick R GlassJames R Friend
Dec 12, 2012·Journal of Biomedical Optics·Victor YashunskyDan Davidov
Oct 20, 2011·Cellular and Molecular Life Sciences : CMLS·Tetiana Kurkina, Kannan Balasubramanian
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Nov 30, 2010·Expert Opinion on Drug Discovery·Ye Fang
Jan 28, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Siddarth ChandrasekaranYe Fang
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Apr 1, 2009·Optics Express·Björn AgnarssonKristjan Leosson
Nov 3, 2017·The Review of Scientific Instruments·Vladislav LirtsmanDan Davidov
Feb 12, 2013·Chemical Communications : Chem Comm·Muhammad H M SallehJonathan M Cooper
Nov 24, 2011·Optics Express·Björn AgnarssonKristjan Leosson
Jul 29, 2009·Journal of Receptor and Signal Transduction Research·Jeremy J Ramsden, Robert Horvath

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