PMID: 9439646Jan 24, 1998Paper

Multidimensional flow-cytometric analysis of dendritic cells in peripheral blood of normal donors and cancer patients

Cancer Immunology, Immunotherapy : CII
C A SavaryJ H Rex

Abstract

We studied the potential of multidimensional flow cytometry to evaluate the frequency and maturation/activation status of dendritic cells in minimally manipulated peripheral blood mononuclear cell preparations (i.e., only separated on Ficoll-Hypaque) of normal donors and cancer patients. A rare subset of HLA-DR+ leukocytes (less than 1% mononuclear cells) was detected in blood of normal donors that displayed all the features of dendritic cells: these cells had high forward-light-scatter characteristics and coexpressed CD4, CD86 and CD54 surface antigens, but lacked the lineage-associated surface markers of T cells, B cells, monocytes, granulocytes or NK i.e. they were CD3-, CD19-, CD20-, CD14-, CD11b-, CD16-, CD56-). These physical and phenotypic properties were virtually identical to those of immunomagnetically sorted leukocytes characterized as dendritic-cells on the basis of morphology, phenotype and high stimulatory activity in allogeneic mixed-lymphocyte cultures. Using this flow-cytometric approach we observed that the frequency of dendritic cell-like cells in peripheral blood mononuclear cell specimens of cancer patients receiving chemotherapy alone or those recovering from stem cell transplantation was significantly low...Continue Reading

Citations

May 16, 2007·Cancer Immunology, Immunotherapy : CII·Sandra TuyaertsAude Bonehill
Jan 23, 2004·Journal of Immunological Methods·S VuckovicD N J Hart
Aug 23, 2002·Journal of Immunological Methods·Sandra TuyaertsKris Thielemans
Sep 16, 2000·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·H HasebeM Eriguchi
Feb 28, 2002·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·B Melichar, R S Freedman
Jul 10, 2013·The Journal of Experimental Medicine·Alberto Pinzon-CharryMichael F Good
Jan 19, 2002·Journal of Hematotherapy & Stem Cell Research·J VakkilaU M Saarinen-Pihkala
Jun 21, 2001·In Vitro Cellular & Developmental Biology. Animal·C A SavaryJ H Rex
Dec 16, 2005·Neoplasia : an International Journal for Oncology Research·Alberto Pinzon-CharryJosé Alejandro López
Dec 16, 2005·Neoplasia : an International Journal for Oncology Research·Alberto Pinzon-CharryJosé Alejandro López
Mar 3, 2007·Transplant Immunology·Jeroen H GerritsNicole M van Besouw
Mar 11, 2004·Clinical and Experimental Immunology·J VakkilaU M Saarinen-Pihkala
Jan 1, 1999·Cytotherapy·A P Gee
Jun 19, 2004·Cytotherapy·T H DoI M Svane
Dec 3, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T AgrawalA Mittal
May 18, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·M A SchnellJ M Wilson
Oct 10, 2007·British Journal of Cancer·A Pinzon-CharryJ A López
Dec 14, 2017·Cytometry. Part B, Clinical Cytometry·Evdokiya ReshetnikovaLudmila Mechetina
Apr 25, 2001·Bone Marrow Transplantation·M GrazziuttiC A Savary
Sep 5, 2002·Bone Marrow Transplantation·D DamianiR Fanin
Oct 19, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Bohuslav Melichar

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