Multidimensional Proteomic Approach of Endothelial Progenitors Demonstrate Expression of KDR Restricted to CD19 Cells.

Stem Cell Reviews and Reports
Coralie L GuerinDavid M Smadja

Abstract

Endothelial progenitor cells (EPCs) are involved in vasculogenesis and cardiovascular diseases. However, the phenotype of circulating EPCs remains elusive but they are more often described as CD34+KDR+. The aim of the study was to extensively characterize circulating potential vasculogenic stem cell candidates in two populations of patients with cardiovascular disease by powerful multidimensional single cell complementary cytometric approaches (mass, imaging and flow). We identified cellular candidates in one patient before and after bioprosthetic total artificial heart implantation and results were confirmed in healthy peripheral and cord blood by mass cytometry. We also quantified cellular candidates in 10 patients with different COVID-19 severity. Both C-TAH implantation and COVID-19 at critical stage induce a redistribution of circulating CD34+ and CD19+ sub-populations in peripheral blood. After C-TAH implantation, circulating CD34+ progenitor cells expressed c-Kit stem marker while specific subsets CD34+CD133-/+CD45-/dimc-Kit+KDR- were mobilized. KDR was only expressed by CD19+ B-lymphocytes and CD14+ monocytes subpopulations in circulation. We confirmed by mass cytometry this KDR expression on CD19+ in healthy peripheral...Continue Reading

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Citations

Mar 3, 2021·Stem Cell Reviews and Reports·Grégoire DetricheDavid M Smadja
Jun 30, 2021·Angiogenesis·David M SmadjaArjan W Griffioen
Nov 2, 2021·Stem Cells Translational Medicine·Sarah E J ChambersReinhold J Medina

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Methods Mentioned

BETA
density gradient centrifugation
FACS
flow cytometry
flow

Clinical Trials Mentioned

NCT04333368

Software Mentioned

Cytosplore
SPADE
R studio
ImageStreamX
IDEAS
FlowJo
Carmat
Cytobank
SARCODO
HSNE

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