Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species.

The Journal of Biological Chemistry
Jonathan M ConwayRobert Kelly

Abstract

The genome of the extremely thermophilic bacterium Caldicellulosiruptor kronotskyensisencodes 19 surface layer (S-layer) homology (SLH) domain-containing proteins, the most in any Caldicellulosiruptorspecies genome sequenced to date. These SLH proteins include five glycoside hydrolases (GHs) and one polysaccharide lyase, the genes for which were transcribed at high levels during growth on plant biomass. The largest GH identified so far in this genus, Calkro_0111 (2,435 amino acids), is completely unique toC. kronotskyensisand contains SLH domains. Calkro_0111 was produced recombinantly inEscherichia colias two pieces, containing the GH16 and GH55 domains, respectively, as well as putative binding and spacer domains. These displayed endo- and exoglucanase activity on the β-1,3-1,6-glucan laminarin. A series of additional truncation mutants of Calkro_0111 revealed the essential architectural features required for catalytic function. Calkro_0402, another of the SLH domain GHs inC. kronotskyensis, when produced inE. coli, was active on a variety of xylans and β-glucans. Unlike Calkro_0111, Calkro_0402 is highly conserved in the genus Caldicellulosiruptorand among other biomass-degrading Firmicutes but missing from Caldicellulosirup...Continue Reading

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Citations

May 10, 2017·Applied and Environmental Microbiology·Amanda M Williams-RhaesaMichael W W Adams
May 22, 2016·Applied and Environmental Microbiology·Gina L LipscombMichael W W Adams
Jul 2, 2016·Biotechnology for Biofuels·Darrian TalamantesRenaud Berlemont
Nov 23, 2019·Applied Microbiology and Biotechnology·Ahmet H BadurChristopher V Rao
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Andika SidarPeter J Punt
May 26, 2018·Extremophiles : Life Under Extreme Conditions·Amanda M Williams-RhaesaMichael W W Adams
Feb 17, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·James A CountsRobert M Kelly
Jul 31, 2019·Extremophiles : Life Under Extreme Conditions·Laura L LeeRobert M Kelly
Jan 12, 2021·Applied Microbiology and Biotechnology·Daniel Mehabie MulualemAoife Boyd
Dec 15, 2020·International Journal of Biological Macromolecules·Nisha SinghMunish Puri
Mar 16, 2019·Current Opinion in Biotechnology·James R CrosbyRobert M Kelly
Mar 21, 2017·Current Opinion in Biotechnology·Christopher T StraubRobert M Kelly
Mar 9, 2021·Environmental Microbiology Reports·Ryan G BingRobert M Kelly
Jul 17, 2018·Metabolic Engineering Communications·Amanda M Williams-RhaesaMichael W W Adams

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