Multidrug resistance-associated protein 2 is involved in the efflux of Aconitum alkaloids determined by MRP2-MDCKII cells

Life Sciences
Peimin DaiZhong-Qiu Liu

Abstract

Aconitum alkaloids mainly contain highly toxic aconitine (AC), mesaconitine (MA), and hypaconitine (HA) and less toxic benzoylaconine (BAC), benzoylmesaconine (BMA), benzoylhypaconine (BHA), aconine, mesaconine, and hypaconine. The efflux transporters including P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and multidrug resistance-associated protein 2 (MRP2) can efflux toxicants to prevent poisoning. Our previous publication has proved that P-gp and BCRP contributed to the efflux of AC, MA and HA, which is demonstrated in the human colonic adenocarcinoma cell lines (Caco-2 cells), Mardin-Darby canine kidney cell lines transfected with MDR1 or BCRP (MDR1-MDCKII and BCRP-MDCKII cells). However, the role of MRP2 remains uncertain. The MRP2-MDCKII cells were used to determine the efflux ratios (Er) and intracellular amounts of Aconitum alkaloids. In addition, the importance of MRP2 was further investigated with or without the MRP2 inhibitor, LTC4. The Er values of AC, MA, HA, BAC, BMA and BHA in MRP2-MDCKII cells (6.4 ± 0.3, 5.9 ± 0.5, 2.2 ± 0.2, 1.6 ± 0.3, 1.7 ± 0.2 and 1.9 ± 0.2 respectively) were significantly higher than those in MDCKII cells, which were close to 1. In the presence of LTC4, the Er values of AC,...Continue Reading

References

Jun 1, 1979·The Tohoku Journal of Experimental Medicine·H SatoH Hikino
Nov 1, 1981·Journal of Ethnopharmacology·N G Bisset
Dec 1, 1993·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Y SuzukiM Noguchi
Oct 7, 1998·Progress in Neurobiology·A Ameri
Oct 6, 2006·International Journal of Legal Medicine·An A Van LandeghemMichel H A Piette
Feb 21, 2008·Journal of Forensic Sciences·Ravi PullelaEdward W Randell
Aug 5, 2009·Journal of Ethnopharmacology·Judith SinghuberBrigitte Kopp
Nov 3, 2009·Human & Experimental Toxicology·Thomas Y K Chan
Nov 26, 2010·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Ling YeZhongqiu Liu
Jun 7, 2011·Forensic Science International : Synergy·Qian LiuYan Liu
Jul 16, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Zhen YangMing Hu
Apr 9, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Cuiping YangZhenqing Zhang

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Citations

Oct 27, 2015·Future Medicinal Chemistry·Elisabeta BaiceanuPierre Falson
Sep 1, 2015·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Victor KueteThomas Efferth
Feb 18, 2017·Clinical Pharmacokinetics·Anton IvanyukThierry Buclin
Apr 2, 2021·The Journal of Pharmacy and Pharmacology·Lu GaoBaojian Wu

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