Multidrug resistance in Aspergillus nidulans involves novel ATP-binding cassette transporters

Molecular & General Genetics : MGG
G Del SorboM A De Waard

Abstract

Two single-copy genes, designated atrA and atrB (ATP-binding cassette transporter A and B), were cloned from the filamentous fungus Aspergillus nidulans and sequenced. Based on the presence of conserved motifs and on hydropathy analysis, the products encoded by atrA and atrB can be regarded as novel members of the ATP-binding cassette (ABC) superfamily of membrane transporters. Both products share the same topology as the ABC transporters PDR5 and SNQ2 from Saccharomyces cerevisiae and CDR1 from Candida albicans, which are involved in multidrug resistance of these yeasts. Significant homology also occurs between the ATP-binding cassettes of AtrA and AtrB, and those of mammalian ABC transporters (P-glycoproteins). The transcription of atrA and, in particular, atrB in mycelium of A. nidulans is strongly enhanced by treatment with several drugs, including antibiotics, azole fungicides and plant defense toxins. The enhanced transcription is detectable within a few minutes after drug treatment and coincides with the beginning of energy-dependent drug efflux activity, reported previously in the fungus for azole fungicides. Transcription of the atr genes has been studied in a wild-type and in a series of isogenic strains carrying the ...Continue Reading

Citations

Jun 14, 2002·The Plant Journal : for Cell and Molecular Biology·Sybille van den BrûleCheryl C Smart
Jul 24, 2002·European Journal of Biochemistry·Stephan J A HesseJaap Visser
Nov 18, 1998·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·M KolaczkowskiA Goffeau
Jan 20, 2004·Plant Physiology·Martha C AlvaradoLászló M Szabados
Nov 19, 2002·Antimicrobial Agents and Chemotherapy·Lute-Harm ZwiersMaarten A De Waard
Apr 16, 2009·Clinical Microbiology Reviews·Richard D CannonBrian C Monk
Mar 18, 2010·BMC Genomics·Andriy Kovalchuk, Arnold J M Driessen
Aug 5, 2008·Molecular Genetics and Genomics : MGG·Ilka BraumannFrank Kempken
Feb 14, 2006·Pest Management Science·Maarten A de WaardLute-Harm Zwiers
Dec 20, 2000·The Journal of Infection·C B MooreD W Denning
Feb 27, 2003·Journal of Veterinary Pharmacology and Therapeutics·H Vanden BosscheF Rochette
Jul 5, 2005·Expert Opinion on Investigational Drugs·L E Lawrence, J F Barrett
Aug 24, 2000·Fungal Genetics and Biology : FG & B·G Del SorboM A De Waard
Jun 24, 1999·Acta Microbiologica Et Immunologica Hungarica·J F Peberdy

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