Multidrug resistance in breast cancer cells during epithelial-mesenchymal transition is modulated by breast cancer resistant protein

Chinese Journal of Cancer
Wei-Juan ChenWen-Tong Li

Abstract

Epithelial-mesenchymal transition (EMT) not only initiates invasion and metastasis of tumors, but also induces multidrug resistance in tumor cells. Our experiment analyzed the dependability between breast cancer resistant protein (BCRP) and EMT in breast cancer to explore the effect of EMT on BCRP-mediated multidrug resistance. The expressions of BCRP and transcription inhibitor Snai1 (Snail) in breast cancer were detected by immunohistochemistry. The eukaryotic expression vector pCDNA3.1-Snail was constructed and then transfected into human breast cancer cell line MCF-7. Snail, epithelial marker gene E-cadherin, interstitial marker gene Vimentin, multidrug resistance protein BCRP, and relative drug resistance were measured by immunofluorescence, Western blot, real-time polymerase chain reaction (PCR), and MTT assay. Immunohistochemistry showed that Snail was highly correlated with BCRP in breast cancer. Immunofluorescence, Western blot, real-time PCR revealed that compared with parent cell MCF-7, after transfected with Snail, the expression of E-cadherin in MCF-7 decreased, but Snail, Vimentin, and BCRP increased. MTT displayed that the relative drug resistance increased to 9.93. After transfected with eukaryotic expression ve...Continue Reading

Citations

Dec 21, 2012·The Journal of Biological Chemistry·Zixing LiuMing Tan
Jul 24, 2015·International Journal of Oncology·Jing HuangGuosheng Ren
Sep 4, 2016·Pathology Oncology Research : POR·Golnoush Dehbashi BehbahaniSadegh Babashah
Apr 18, 2018·British Journal of Pharmacology·Ana Isabel Fraguas-SánchezAna Isabel Torres-Suárez
Nov 2, 2019·Nature Reviews. Cancer·Elizabeth D WilliamsErik W Thompson
Jul 24, 2018·Oncotarget·Atrayee BhattacharyaArchana Dhasarathy
Sep 3, 2020·Cell Biology and Toxicology·Yuanming JingZongliang Huang
Feb 25, 2019·Breast Cancer Research and Treatment·Emily A BossartSteffi Oesterreich
Dec 17, 2014·Cancer Research·Janneke E JaspersSven Rottenberg
Jan 11, 2021·Advances in Biological Regulation·Pratibha MalhotraMarco Falasca
Feb 5, 2021·OncoTargets and Therapy·Shanshan YuanXin Zhang
Jul 18, 2020·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Nuray ErinThomas Efferth
Aug 26, 2021·Journal of Cellular Physiology·Ishita S HiremathManoj Garg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.

Related Papers

Biomedical and Environmental Sciences : BES
Jian-Hui YuanZhi-Xiong Zhuang
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
Jian-Hui YuanZhi-Xiong Zhuang
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Ian F FaneyteMarc J van de Vijver
© 2021 Meta ULC. All rights reserved