Multidrug-resistance phenotype of a subpopulation of T-lymphocytes without drug selection.

Experimental Cell Research
A A NeyfakhA R Kazarov

Abstract

Multidrug-resistant (MDR) cells demonstrate the increased activity of the membrane transport system performing efflux of diverse lipophylic drugs and fluorescent dyes from the cells. In order to detect MDR cells we have developed a simple test consisting of three steps: staining of the cells with fluorescent dye rhodamine 123, incubation in the dye-free medium and, finally, detection by fluorescence microscopy of the cells that have lost accumulated dye. The experiments with B-lymphoma cell lines with different degrees of MDR have shown that the cell fluorescence after the poststaining incubation is indeed inversely proportional to the degree of resistance. Application of this testing procedure to normal human or mouse leukocytes revealed the presence of the cells rapidly losing the dye in these populations. Cell fractionation experiments have shown that there are T-lymphocytes (most T-killers/suppressors and a part of T-helpers) that demonstrate rapid efflux of rhodamine 123. This characteristic was detected also in T-killer clones and cell line and in some T-lymphomas. The inhibitors of the MDR transport system, reserpine and verapamil, blocked the efflux of the dye from these cells. Rhodamine-losing T-lymphoma contained larg...Continue Reading

Citations

Jan 1, 1993·Cytotechnology·U A Germann
Jan 1, 1993·Cancer Immunology, Immunotherapy : CII·A S ChongJ S Coon
Dec 7, 2007·Pflügers Archiv : European journal of physiology·Raphael C ValenteMárcia A M Capella
Oct 1, 1991·Human Immunology·J S CoonH M Gebel
Jun 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J P MarieR Zittoun
Jun 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M BaudardM M Gottesman
Jul 25, 2000·Developmental and Comparative Immunology·M D EisenbraunR A Miller
Jul 1, 2003·Clinical and Applied Immunology Reviews·Shona S PendseMarkus H Frank
Aug 27, 2011·Journal of the American Chemical Society·Hilda A NamanjaJean Chmielewski
Nov 20, 1998·The Journal of Pharmacy and Pharmacology·M KuniharaM Takano
Nov 16, 2001·Antimicrobial Agents and Chemotherapy·H AndoT Hasegawa
Oct 3, 2015·Antiviral Research·Alessandro DalpiazElisabetta Gavini
Jan 7, 2006·Phytotherapy Research : PTR·Varaporn Buraphacheep JunyaprasertMikihisa Takano
May 27, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Suk W ParkEugene Mechetner
Sep 1, 1992·Leukemia & Lymphoma·A F List, C M Spier
Aug 3, 2002·Leukemia & Lymphoma·Dorina M van der KolkEdo Vellenga
Apr 11, 2014·Molecular Pharmaceutics·Alessandro DalpiazSanto Scalia
May 1, 1995·European Journal of Immunology·H R MacDonaldJ C Cerottini
Dec 1, 1993·Leukemia Research·B DrenouR Fauchet
Sep 20, 2018·Clinical and Experimental Immunology·J R FergussonL J Walker
Jun 17, 2009·Hypertension·João Marcos A DelouMárcia A M Capella
Nov 13, 2004·The Journal of Antimicrobial Chemotherapy·J FordD J Back
Sep 16, 2008·The Journal of Pharmacology and Experimental Therapeutics·Miriam HulsRosalinde Masereeuw
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M D EisenbraunR A Miller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.