Multielectrode recordings from auditory neurons in the brain of a small grasshopper

Journal of Neuroscience Methods
Mit Balvantray BhavsarAndreas Stumpner

Abstract

Grasshoppers have been used as a model system to study the neuronal basis of insect acoustic behavior. Auditory neurons have been described from intracellular recordings. The growing interest to study population activity of neurons has been satisfied so far with artificially combining data from different individuals. We for the first time used multielectrode recordings from a small grasshopper brain. We used three 12μm tungsten wires (combined in a multielectrode) to record from local brain neurons and from a population of auditory neurons entering the brain from the thorax. Spikes of the recorded units were separated by sorting algorithms and spike collision analysis. The tungsten wires enabled stable recordings with high signal to noise ratio. Due to the tight temporal coupling of auditory activity to the stimulus spike collisions were frequent and collision analysis retrieved 10-15% of additional spikes. Marking the electrode position was possible using a fluorescent dye or electrocoagulation with high current. Physiological identification of units described from intracellular recordings was hard to achieve. 12μm tungsten wires gave a better signal to noise ratio than 15μm copper wires previously used in recordings from bees...Continue Reading

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Feb 21, 2019·Annual Review of Neuroscience·Christa A BakerMala Murthy
Feb 18, 2021·Proceedings. Biological Sciences·Jan ClemensMichael S Reichert
Nov 27, 2020·Frontiers in Behavioral Neuroscience·Alejandro CámeraDaniel Tomsic

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