Multiepitope-Based Subunit Vaccine Design and Evaluation against Respiratory Syncytial Virus Using Reverse Vaccinology Approach

Vaccines
Muhammad Tahir Ul QamarNazamid Saari

Abstract

Respiratory syncytial virus (RSV) is primarily associated with respiratory disorders globally. Despite the availability of information, there is still no competitive vaccine available for RSV. Therefore, the present study has been designed to develop a multiepitope-based subunit vaccine (MEV) using a reverse vaccinology approach to curb RSV infections. Briefly, two highly antigenic and conserved proteins of RSV (glycoprotein and fusion protein) were selected and potential epitopes of different categories (B-cell and T-cell) were identified from them. Eminently antigenic and overlapping epitopes, which demonstrated strong associations with their respective human leukocyte antigen (HLA) alleles and depicted collective ~70% coverage of the world's populace, were shortlisted. Finally, 282 amino acids long MEV construct was established by connecting 13 major histocompatibility complex (MHC) class-I with two MHC class-II epitopes with appropriate adjuvant and linkers. Adjuvant and linkers were added to increase the immunogenic stimulation of the MEV. Developed MEV was stable, soluble, non-allergenic, non-toxic, flexible and highly antigenic. Furthermore, molecular docking and molecular dynamics (MD) simulations analyses were carried ...Continue Reading

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Citations

Sep 18, 2020·Infectious Diseases of Poverty·Muhammad Tahir Ul QamarLing-Ling Chen
Nov 10, 2020·Journal of Biomolecular Structure & Dynamics· JyotishaInsaf Ahmed Qureshi
Nov 18, 2020·Journal of Molecular Liquids·Sajjad AhmadMuzammil Hasan Najmi
Mar 6, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Marvah MahmoodUsman Ali Ashfaq
Oct 7, 2021·Scientific Reports·Adewale Oluwaseun FadakaMervin Meyer

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Software Mentioned

GalaxyRefining2
GENBANK
GROMACS
- tool
AllerTOP
Blastp analysis
ProtParam
NetMHCIIpan
PSIPRED
Ellipro

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