Multiepitope fusion antigen induces broadly protective antibodies that prevent adherence of Escherichia coli strains expressing colonization factor antigen I (CFA/I), CFA/II, and CFA/IV

Clinical and Vaccine Immunology : CVI
Xiaosai RuanWeiping Zhang

Abstract

Diarrhea is the second leading cause of death in children younger than 5 years and continues to be a major threat to global health. Enterotoxigenic Escherichia coli (ETEC) strains are the most common bacteria causing diarrhea in developing countries. ETEC strains are able to attach to host small intestinal epithelial cells by using bacterial colonization factor antigen (CFA) adhesins. This attachment helps to initiate the diarrheal disease. Vaccines that induce antiadhesin immunity to block adherence of ETEC strains that express immunologically heterogeneous CFA adhesins are expected to protect against ETEC diarrhea. In this study, we created a CFA multiepitope fusion antigen (MEFA) carrying representative epitopes of CFA/I, CFA/II (CS1, CS2, and CS3), and CFA/IV (CS4, CS5, and CS6), examined its immunogenicity in mice, and assessed the potential of this MEFA as an antiadhesin vaccine against ETEC. Mice intraperitoneally immunized with this CFA MEFA exhibited no adverse effects and developed immune responses to CFA/I, CFA/II, and CFA/IV adhesins. Moreover, after incubation with serum of the immunized mice, ETEC or E. coli strains expressing CFA/I, CFA/II, or CFA/IV adhesins were significantly inhibited in adherence to Caco-2 ce...Continue Reading

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Citations

Jul 3, 2015·Clinical and Vaccine Immunology : CVI·Weiping Zhang, David A Sack
Jun 20, 2020·Gut Microbes·Hyesuk SeoWeiping Zhang
Jul 11, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·J Daniel Dubreuil
Apr 7, 2021·Protein and Peptide Letters·Sasikumar SabnaSyed Imteyaz Alam

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