Multifactor analysis on the effect of collagen concentration, cross-linking and fiber/pore orientation on chemical, microstructural, mechanical and biological properties of collagen type I scaffolds

Materials Science & Engineering. C, Materials for Biological Applications
Edward SuescaMarta R Fontanilla

Abstract

This work evaluates the effect of processing variables on some physicochemical and mechanical properties of multi- and unidirectional laminar collagen type I scaffolds. The processing variables considered in this study included microstructure orientation (uni- and multidirectional fiber/pore controlled by freeze-drying methodology), cross-linking (chemical - using genipin and glutaraldehyde, and physical - using a dehydrothermal method), and collagen concentration (2, 5 and 8mg/ml). The biocompatibility of the scaffolds obtained in each of the evaluated manufacturing processes was also assessed. Despite previous research on collagen-based platforms, the effects that these processing variables have on the properties of collagen scaffolds are still not completely understood. Unidirectional scaffolds presented higher resistance to failure under stress than multidirectional ones. The cross-linking degree was found to decrease when the concentration of collagen increased whilst using chemical cross-linkers, and to increase with the concentration of collagen for the dehydrothermal cross-linked scaffolds. Pore orientation indexes of both unidirectional and multidirectional scaffolds were not influenced by collagen concentration. Cross...Continue Reading

Citations

Nov 6, 2018·Drug Delivery and Translational Research·Liliana Gil-CifuentesMarta R Fontanilla
May 14, 2020·Journal of Biomaterials Science. Polymer Edition·Amjad AlaghaSahar Alhariri
Nov 4, 2020·Journal of Functional Biomaterials·Nunzia GalloLuca Salvatore
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Diana MillánMarta R Fontanilla
Mar 17, 2020·International Journal of Biological Macromolecules·Luca SalvatoreAlessandro Sannino
Mar 7, 2021·Polymers·Gustavo A Rico-LlanosRick Visser
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Delu ZhaoMiao Yin
Aug 29, 2021·Acta Biomaterialia·Arezoo DadashzadehChristiani A Amorim

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