Multifactorial analysis of affinity-mass spectrometry data from serum protein samples: a strategy to distinguish patients with preeclampsia from matching control individuals.

Journal of the American Society for Mass Spectrometry
Ulrich PecksMichael O Glocker

Abstract

A multifactorial differential analysis of serum proteins using mass spectrometry distinguished samples from pregnant women with severe early-onset preeclampsia (n = 11) from those of control individuals with uneventful pregnancies (n = 13). Serum proteins were fractionated by either their affinities to reversed-phase material coated magnetic beads or by fractionated precipitation. The on-average most abundant ion signals were observed at m/z 9390, 9103, and 8886. The best differentiating ion signals between the two sample groups were found at m/z 13,715, 13,834, and 13,891. The normalized intensities of these ion signals were on-average lower in the preeclampsia group than in the control group. The six ion signal intensities enabled sorting of the individual spectra with high accuracy. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that a protein band migrating just above the 14 kDa marker band contained transthyretin (P02766; M(r) (avg.): 13,761). Densitometric analysis of the transthyretin bands showed lower intensities in the preeclampsia samples with respect to those of the controls. Nephelometric analysis of the serum samples determined the mean concentration of transthyretin in the pr...Continue Reading

References

Dec 15, 1982·European Journal of Biochemistry·P W DicksonG Schreiber
Dec 1, 1995·Computer Methods and Programs in Biomedicine·F SchoonjansF H Comhaire
May 1, 1996·Reviews of Reproduction·F Lyall, I A Greer
May 8, 1998·Obstetrics and Gynecology·W HolzgreveS Hahn
Jul 20, 1999·American Journal of Obstetrics and Gynecology·J AquilinaK Harrington
Jan 29, 2000·The American Journal of Clinical Nutrition·V SapinV Azaïs-Braesco
Oct 14, 2000·Journal of Perinatal Medicine·W RathJ W Dudenhausen
Feb 24, 2001·Lancet·J M Roberts, D W Cooper
Aug 15, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·F ReisterB Huppertz
Mar 22, 2003·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Nick A BersingerChristopher W Redman
Jun 12, 2003·Obstetrics and Gynecology·Aravinthan CoomarasamyKhalid Saeed Khan
Aug 12, 2003·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Peter von DadelszenJames M Roberts
Aug 23, 2003·Biostatistics·Nancy A Obuchowski, Xiao-Hua Zhou
Feb 7, 2004·The New England Journal of Medicine·Richard J LevineS Ananth Karumanchi
May 7, 2004·The New England Journal of Medicine·Vassilis TsatsarisJean Michel Foidart
Jun 3, 2004·Electrophoresis·Giovanni CandianoPier Giorgio Righetti
Sep 8, 2004·Proteomics·Masahiro SeikeSetsuo Hirohashi
Dec 2, 2004·Obstetrics and Gynecology·Agustin Conde-AgudeloMarshall Lindheimer
Jan 25, 2005·BJOG : an International Journal of Obstetrics and Gynaecology·Charl OettleDebbie Grové
Dec 24, 2005·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·K H NicolaidesH S Cuckle
Apr 4, 2006·American Journal of Obstetrics and Gynecology·José VillarUNKNOWN World Health Organization Antenatal Care Trial Research Group
May 2, 2006·American Journal of Obstetrics and Gynecology·Irene CetinAlberto Mantovani
Jul 14, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J C HeitnerM O Glocker
Sep 8, 2006·The New England Journal of Medicine·Richard J LevineUNKNOWN CPEP Study Group
Mar 3, 2007·The Journal of Clinical Investigation·Tomas VaisarJay W Heinecke
Nov 3, 2007·BMJ : British Medical Journal·Leanne BellamyDavid J Williams
Jun 27, 2008·Briefings in Functional Genomics & Proteomics·Mu WangDawn P G Brown
Aug 30, 2008·Journal of Lipid Research·Kelly R AtkinsonUNKNOWN SCOPE Consortium
Aug 30, 2008·Fetal Diagnosis and Therapy·Berthold HuppertzHamutal Meiri
Apr 4, 2009·Journal of Spinal Disorders & Techniques·Luc DuongHubert Labelle
Apr 30, 2009·The Journal of Clinical Endocrinology and Metabolism·Kelly A LandersKerry Richard
Jan 1, 2009·Proteomics. Clinical Applications·Magnus PalmbladRainer Cramer

❮ Previous
Next ❯

Citations

Apr 20, 2011·Journal of Neurotrauma·Joanna DietzelAlexander Dressel
May 27, 2015·International Journal of Molecular Sciences·Kai P LawPhilip N Baker
Jan 22, 2011·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Ulrich PecksWerner Rath
May 16, 2012·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Ulrich PecksMichael O Glocker
Jan 29, 2013·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Arrigo FruscalzoFrancesco Curcio
Oct 2, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Toralf ReimerBernd Gerber
Sep 25, 2014·Archives of Gynecology and Obstetrics·A FruscalzoL Driul
Jan 7, 2015·Journal of the American Society for Mass Spectrometry·Yelena YefremovaMichael O Glocker
Oct 21, 2016·Archives of Gynecology and Obstetrics·Johannes StubertToralf Reimer
May 9, 2018·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Lina A Dahabiyeh
May 31, 2019·Molecular & Cellular Proteomics : MCP·Bright D DanquahMichael O Glocker
Jun 19, 2014·Experimental and Therapeutic Medicine·Lei ZhuZhenyu Zhang
Dec 11, 2019·Atherosclerosis. Supplements·Christine ContiniKarl Winkler
Aug 1, 2019·Molecular & Cellular Proteomics : MCP·Bright D DanquahMichael O Glocker

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.