Multifunctional Analysis of CD4+ T-Cell Response as Immune-Based Model for Tuberculosis Detection

Journal of Immunology Research
Miriam LichtnerClaudio M Mastroianni

Abstract

Mono- and multifunctional specific CD4(+) and CD8(+) T-cell responses were evaluated to improve the immune-based detection of active tuberculosis (TB) and latent infection (LTBI). We applied flow cytometry to investigate cytokines profile (IFN-γ, TNF-α, and IL-2) of T cells after stimulation with TB antigens in 28 TB-infected subjects (18 active TB and 10 LTBI) and 10 uninfected controls. Cytokines production by CD4(+) T cells at single-cell levels was higher in TB-infected subjects than uninfected controls (P < 0.0001). Assigning to activated CD4(+) T cells, producing any of the three cytokines, a cut-off >0.45%, it was possible to differentiate TB-infected (>0.45%) by uninfected subjects (<0.45%). Among TB-infected subjects, the frequencies of multifunctional CD4(+) T cells, simultaneously producing all 3 cytokines, are lower in active TB than LTBI subjects (P = 0.003). Thus, assigning to triple-positive CD4(+) T cells a cut-off <0.182%, TB-infected individuals could be classified as active TB subjects (<0.182%) or LTBI subjects (>0.182%). The magnitude of CD8(+) T-cell responses showed no differences between active TB and LTBI. Multifunctional CD4(+) T-cell responses could have the potential to identify at single time point ...Continue Reading

References

Sep 13, 2003·American Journal of Respiratory and Critical Care Medicine·Deborah A LewinsohnDavid M Lewinsohn
Apr 20, 2006·Nature Reviews. Immunology·Giuseppe Pantaleo, Alexandre Harari
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kerry A MillingtonAjit Lalvani
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emily K ForbesElma Z Tchilian
Feb 19, 2009·European Journal of Immunology·Jayne S SutherlandMartin O C Ota
Jun 25, 2009·BMC Infectious Diseases·Valeria SargentiniRoberto Nisini
Oct 27, 2009·Nature Reviews. Microbiology·Clifton E BarryDouglas Young
Nov 11, 2009·Cytometry. Part B, Clinical Cytometry·Dong Il Won, Jung Ran Park
Apr 27, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Keertan DhedaYing Zhang
Jun 12, 2010·European Journal of Immunology·Nadia CaccamoFrancesco Dieli
Feb 15, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joshua T MattilaJoAnne L Flynn
Jul 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cheryl L DayWillem A Hanekom
Nov 11, 2011·American Journal of Respiratory and Critical Care Medicine·Christina LancioniUNKNOWN Tuberculosis Research Unit
Jan 16, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susanna CommandeurTom H M Ottenhoff
Mar 7, 2013·The Journal of Infection·Elisa PetruccioliDelia Goletti
Jan 8, 2014·Clinical Microbiology Reviews·Madhukar PaiNiaz Banaei
Nov 2, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Virginie RozotAlexandre Harari

❮ Previous
Next ❯

Citations

Jun 26, 2015·Medical Microbiology and Immunology·Ilaria SauzulloClaudio M Mastroianni
Jun 2, 2016·American Journal of Respiratory and Critical Care Medicine·Katalin A WilkinsonCatherine Riou
Sep 30, 2016·Oncotarget·Ilana C van RensburgAndre G Loxton
Aug 11, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Natalie StricklandCatherine Riou
Oct 5, 2018·Frontiers in Immunology·Riccardo ArrigucciMaria Laura Gennaro
Sep 12, 2018·Nature Microbiology·Michelle A SallinDaniel L Barber
Feb 20, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Muthya Pragun AcharyaVijaya Satchidanandam

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
X-ray
FACS

Software Mentioned

FlowJo

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.