Multifunctional Dendrimer-Entrapped Gold Nanoparticles for Labeling and Tracking T Cells Via Dual-Modal Computed Tomography and Fluorescence Imaging.

Biomacromolecules
Meixiu ChenXiangyang Shi

Abstract

Nanosystems for monitoring and tracking T cells provide an important basis for evaluating the functionality and efficacy of T cell-based immunotherapy. To this end, we designed herein an efficient nanoprobe for T cell monitoring and tracking using poly(amidoamine) (PAMAM) dendrimer-entrapped gold nanoparticles (Au DENPs) conjugated with Fluo-4 for dual-mode computed tomography (CT) and fluorescence imaging. In this study, PAMAM dendrimers of generation 5 (G5) were modified with hydroxyl-terminated polyethylene glycol (PEG) and then used to entrap 2.0 nm Au NPs followed by acetylation of the excess amine groups on the dendrimer surface. Subsequently, the calcium ion probe was covalently attached to the dendrimer nanohybrids through the PEG hydroxyl end groups to gain the functional {(Au0)25-G5.NHAc-(PEG)14-(Fluo-4)2} nanoprobe. This nanoprobe had excellent water solubility, high X-ray attenuation coefficient, and good cytocompatibility in the given concentration range, as well as a high T cell labeling efficiency. Confocal microscopy and flow cytometry results demonstrated that the nanoprobe was able to fluorescently sense activated T cells. Moreover, the nanoprobe was able to realize both CT and fluorescence imaging of subcutan...Continue Reading

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Citations

Sep 20, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Haonan LiKui Luo
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Xin YuanXiangyang Shi
Nov 2, 2020·Drug Discovery Today·Ramya ChandrasekaranDon-Hyung Ha
Mar 7, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Serge MignaniJean-Pierre Majoral
May 29, 2021·Radiology. Imaging Cancer·Christina H Liu, Piotr Grodzinski

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