Multifunctional envelope-type nano device (MEND) for organelle targeting via a stepwise membrane fusion process

Methods in Enzymology
Yuma YamadaHideyoshi Harashima

Abstract

A single cell contains a variety of organelles. Included among these organelles are the nucleus that regulates the central dogma, mitochondria that function as an energy plant, the Golgi apparatus that determines the destination of endogenous protein, and others. If it were possible to prepare a nano craft that could specifically target a specific organelle, this would open a new field of research directed toward therapy for various diseases. We recently developed a new concept of "Programmed Packaging," by which we succeeded in creating a multifunctional envelope-type nano device (MEND) as a nonviral gene-delivery system. Our attempts to target certain organelles (nucleus and mitochondria) are described here, mainly focusing on the construction of a tetra-lamellar MEND (T-MEND), and on methods for screening the organelle-specific fusogenic envelope. The critical structural elements of the T-MEND include an organelle-specific membrane-fusogenic inner envelope and a cellular membrane-fusogenic outer envelope. The resulting T-MEND can be utilized to overcome intracellular membrane barriers, since it involves stepwise membrane fusion. To deliver cargos into a target organelle in our strategy, the carriers must fuse with the organe...Continue Reading

Citations

Sep 21, 2013·Expert Opinion on Drug Delivery·Conchita Tros de Ilarduya, Nejat Düzgüneş
Jul 15, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yuma YamadaHideyoshi Harashima
Jan 5, 2016·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yuma Yamada
Nov 5, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yuma Yamada
Oct 6, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yuma Yamada, Hideyoshi Harashima
Jan 5, 2019·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yuma Yamada
May 6, 2015·Bioconjugate Chemistry·Yasuhiro HayashiHideyoshi Harashima

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