Multifunctional Enzyme Packaging and Catalysis in the Qβ Protein Nanoparticle

Biomacromolecules
Jason D FiedlerM G Finn

Abstract

The simultaneous expression in Escherichia coli cells of the Qβ virus-like particle (VLP) capsid protein and protein "cargo" tagged with a positively charged Rev peptide sequence leads to the spontaneous self-assembly of VLPs with multiple copies of the cargo inside. We report the packaging of four new enzymes with potential applications in medicine and chemical manufacturing. The captured enzymes are active while inside the nanoparticle shell and are protected from environmental conditions that lead to free-enzyme destruction. We also describe genetic modifications to the packaging scheme that shed light on the self-assembly mechanism of this system and allow indirect control over the internal packaging density of cargo. The technology was extended to create, via self-assembly, VLPs that simultaneously display protein ligands on the exterior and contain enzymes within. Inverse relationships were observed between the size of both the packaged and externally displayed protein or domains and nanoparticle yield. These results provide a general method for the rapid creation of robust protein nanoparticles with desired catalytic and targeting functionalities.

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Citations

Jul 1, 2020·Advanced Drug Delivery Reviews·Young Hun ChungNicole F Steinmetz
Feb 16, 2021·Biotechnology and Bioprocess Engineering : BBE·Yuanzheng WuHyun-Jae Shin
Jan 16, 2021·Chembiochem : a European Journal of Chemical Biology·Roy A J F OerlemansJan C M van Hest
Aug 14, 2020·Biochemistry·Soumen DasM G Finn
Mar 27, 2019·ACS Nano·Liangjun ZhaoM G Finn
Aug 28, 2021·Current Opinion in Biotechnology·Hye Jin Lim, Dong-Myung Kim
Sep 10, 2021·Biomacromolecules·Antonina NaskalskaJonathan G Heddle
Sep 23, 2021·Journal of the American Chemical Society·Arezoo ShahrivarkevishahiJeremiah J Gassensmith
Aug 22, 2021·Angewandte Chemie·Philipp LohnerClaudia Jessen-Trefzer
Jan 14, 2022·Expert Review of Vaccines·Hunter McFall-Boegeman, Xuefei Huang

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