Multifunctional N-P-doped carbon dots for regulation of apoptosis and autophagy in B16F10 melanoma cancer cells and in vitro imaging applications.

Theranostics
Vivek K BajpaiYoung-Kyu Han

Abstract

Rationale: The present study reports the multifunctional anticancer activity against B16F10 melanoma cancer cells and the bioimaging ability of fluorescent nitrogen-phosphorous-doped carbon dots (NPCDs). Methods: The NPCDs were synthesized using a single-step, thermal treatment and were characterized by TEM, XPS, fluorescence and UV-Vis spectroscopy, and FTIR analysis. The anticancer efficacy of NPCDs was confirmed by using cell viability assay, morphological evaluation, fluorescent live-dead cell assay, mitochondrial potential assay, ROS production, RT-PCR, western-blot analysis, siRNA transfection, and cellular bioimaging ability. Results: The NPCDs inhibited the proliferation of B16F10 melanoma cancer cells after 24 h of treatment and induced apoptosis, as confirmed by the presence of fragmented nuclei, reduced mitochondrial membrane potential, and elevated levels of reactive oxygen species. The NPCDs treatment further elevated the levels of pro-apoptotic factors and down-regulated the level of Bcl2 (B-cell lymphoma 2) that weakened the mitochondrial membrane, and activated proteases such as caspases. Treatment with NPCDs also resulted in dose-dependent cell cycle arrest, as indicated by reduced cyclin-dependent kinase (CDK)...Continue Reading

Citations

Nov 12, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yong XiaLuoting Yu
Feb 9, 2021·Chemical Engineering Journal·Shariq SulemanTejraj M Aminabhavi
Nov 8, 2020·International Journal of Molecular Sciences·Mahendra Pal SinghSun Chul Kang
Mar 7, 2021·International Journal of Molecular Sciences·Mingming SongWenxiang Zhang
Jun 4, 2021·BMC Cancer·Mariana Nobre Farias de FrancaCristiane Bani Corrêa
Dec 16, 2020·ACS Biomaterials Science & Engineering·Kumud Malika TripathiTaeYoung Kim

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Methods Mentioned

BETA
fluorescence imaging
Transmission electron microscopy
ELISA
transfection
Assay
electrophoresis
PCR
thermal treatment

Software Mentioned

ImageJ

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