Multifunctional Nanotherapeutics for the Treatment of neuroAIDS in Drug Abusers

Scientific Reports
Rahul Dev JayantMadhavan Nair

Abstract

HIV and substance abuse plays an important role in infection and disease progression. Further, the presence of persistent viral CNS reservoirs makes the complete eradication difficult. Thus, neutralizing the drug of abuse effect on HIV-1 infectivity and elimination of latently infected cells is a priority. The development of a multi-component [antiretroviral drugs (ARV), latency reactivating agents (LRA) and drug abuse antagonist (AT)] sustained release nanoformulation targeting the CNS can overcome the issues of HIV-1 cure and will help in improving the drug adherence. The novel magneto-liposomal nanoformulation (NF) was developed to load different types of drugs (LRAs, ARVs, and Meth AT) and evaluated for in-vitro and in-vivo BBB transmigration and antiviral efficacy in primary CNS cells. We established the HIV-1 latency model using human astrocyte cells (HA) and optimized the dose of LRA for latency reversal, Meth AT in in-vitro cell culture system. Further, PEGylated magneto-liposomal NF was developed, characterized for size, shape, drug loading and BBB transport in-vitro. Results showed that drug released in a sustained manner up to 10 days and able to reduce the HIV-1 infectivity up to ~40-50% (>200 pg/mL to <100 pg/mL) c...Continue Reading

References

May 1, 1995·Journal of Neuropathology and Experimental Neurology·A NathK R Fowke
Apr 8, 1998·Journal of Neurovirology·M McCarthyC Wood
Nov 24, 1999·Journal of Neuropathology and Experimental Neurology·S F AnF Scaravilli
Jan 31, 2002·Annual Review of Medicine·Joel N BlanksonRobert F Siliciano
Jun 29, 2004·Journal of Virology·Diane M P LawrenceEugene O Major
Dec 8, 2004·Journal of Neuroimmunology·Justin C McArthur
Jan 18, 2006·Retrovirology·Alessandro Marcello
Aug 18, 2006·Current Opinion in Neurology·Avindra Nath, Ned Sacktor
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Iain Crawford AnthonyJeanne Elisabeth Bell
Nov 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eliseo A Eugenin, Joan W Berman
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jeanne E BellIain C Anthony
May 7, 2008·The American Journal of Pathology·Hao LiangWen-Zhe Ho
Jun 26, 2008·PloS One·Tong WangHoward E Gendelman
Nov 10, 2009·AIDS Research and Human Retroviruses·Sima Shelly ToussiHarris Goldstein
Apr 17, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kathryn A LindlKelly L Jordan-Sciutto
Apr 7, 2011·Current Opinion in Neurology·Patrick GannonDennis L Kolson
Apr 13, 2011·PloS One·Michael KovochichJerome A Zack
Jan 10, 2012·Cold Spring Harbor Perspectives in Medicine·Robert F Siliciano, Warner C Greene
Sep 16, 2014·Neuroscience Research·Ashok ChauhanArwa Mujahid Abdullah
Nov 18, 2014·Nature Reviews. Microbiology·Nancie M ArchinDavid M Margolis
Feb 25, 2015·International Journal of Nanomedicine·Rahul Dev JayantMadhavan Nair
Jan 1, 2014·Molecular and Cellular Therapies·Amit Kumar, Georges Herbein
Mar 6, 2016·Advanced Drug Delivery Reviews·Madhavan NairVidya Sagar
Sep 14, 2016·International Journal of Nanomedicine·Venkata Subba Rao AtluriMadhavan Nair

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Citations

Dec 4, 2019·Current Pharmaceutical Biotechnology·Morteza RabieiSteven J P McInnes
Apr 7, 2019·Scientific Reports·Micaela A MacchioneMaria Ángeles Muñoz-Fernández
Jun 24, 2020·Scientific Reports·Asahi TomitakaMadhavan Nair
Sep 19, 2020·International Journal of Molecular Sciences·Katherine E OdegaardSowmya V Yelamanchili
Dec 15, 2020·Expert Opinion on Drug Delivery·Ajeet Kaushik
Feb 2, 2021·International Journal of Nanomedicine·Sree Pooja VarahachalamAjeet Kaushik
Nov 7, 2020·Brain, Behavior, and Immunity·Behnaz LahootiRahul D Jayant
Jun 24, 2020·ACS Nano·Gaurav ChauhanSergio O Martinez-Chapa

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Methods Mentioned

BETA
PMA
ELISA
PCR
Fluorescence microscopy
flow cytometry

Software Mentioned

IDEAS
Amnis ImageStreamX
Prism Pad

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