Multifunctional oligomer incorporation: a potent strategy to enhance the transfection activity of poly(l-lysine)

Biomaterials Science
Shuai LiuTianying Guo

Abstract

Natural polycations, such as poly(l-lysine) (PLL) and chitosan (CS), have inherent superiority as non-viral vectors due to their unparalleled biocompatibility and biodegradability. However, the application was constrained by poor transfection efficiency and safety concerns. Since previous modification strategies greatly weakened the inherent advantages of natural polycations, developing a strategy for functional group introduction with broad applicability to enhance the transfection efficiency of natural polycations without compromising their cationic properties is imperative. Herein, two uncharged functional diblock oligomers P(DMAEL-b-NIPAM) and P(DMAEL-b-Vlm) were prepared from a lactose derivative, N-iso-propyl acrylamide (NIPAM) as well as 1-vinylimidazole (Vlm) and further functionalized with four small ligands folate, glutathione, cysteine and arginine, respectively, aiming to enhance the interactions of complexes with cells, which were quantified utilizing a quartz crystal microbalance (QCM) biosensor, circumventing the tedious material screening process of cell transfection. Upon incorporation with PLL and DNA, the multifunctional oligomers endow the formulated ternary complexes with great properties suitable for trans...Continue Reading

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Citations

May 9, 2012·Journal of Bioscience and Bioengineering·Xiaobo HuangBin Tang
Jun 28, 2017·ACS Applied Materials & Interfaces·Lipeng ZhuLichen Yin
Jun 29, 2017·ACS Applied Materials & Interfaces·Fangfang LiLichen Yin
Oct 25, 2017·Biomaterials Science·Zhao MengWenxin Wang
Apr 10, 2019·Polymers·Vadim V AnnenkovElena N Danilovtseva
Jul 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shuai LiuTianying Guo
May 22, 2018·Chemical Science·Alicia C McGeachyFranz M Geiger
Feb 1, 2019·International Journal of Molecular Sciences·Abdul QadirAirong Qian
Dec 24, 2017·Advanced Drug Delivery Reviews·Zhao MengWenxin Wang
Jul 10, 2017·ACS Biomaterials Science & Engineering·Shuai LiuWenxin Wang
Nov 11, 2017·Biomacromolecules·Jonathan O'Keeffe AhernWenxin Wang
Jun 23, 2018·ACS Applied Materials & Interfaces·Shuai LiuTianying Guo

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