Multifunctional Protein Materials and Microreactors using Low Complexity Domains as Molecular Adhesives

ACS Nano
Lenka FaltovaPaolo Arosio

Abstract

Recent findings indicate that a class of disordered amino acid sequences promotes functional phase transition of biomolecules in nature. Such sequences consist of low complexity domains (LCDs) that are rich in specific amino acids. In this work, we exploit these sequences by conjugating them to soluble globular domains to develop molecular adhesives that enable sensitive, controlled self-assembly of these proteins into supramolecular architectures. In particular, we used the enzyme adenylate kinase and the green fluorescent protein as soluble domains, and we show that the addition of low complexity regions induces the formation of protein particles via a multistep process. This multistep pathway involves an initial liquid-liquid phase transition, which creates protein-rich droplets that mature into protein aggregates over time. These protein aggregates consist of permeable structures that maintain activity and release active soluble proteins. We show that the LCDs dictate specific noncovalent intermolecular interactions and phase properties that are largely independent of the given globular domain. We further demonstrate that this feature, together with the dynamic state of the initial dense liquid phase, allows one to directly...Continue Reading

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Citations

May 1, 2019·Chembiochem : a European Journal of Chemical Biology·Nicolas Martin
Jul 25, 2019·Angewandte Chemie·Miriam LinsenmeierPaolo Arosio
May 16, 2019·ELife·Christoph WeberL Mahadevan
Dec 10, 2020·Nature Communications·Wiggert J AltenburgJan C M van Hest
Feb 16, 2021·ACS Applied Materials & Interfaces·Marleen H M E van StevendaalJan C M van Hest
Feb 3, 2021·Biophysical Chemistry·Fulvio Grigolato, Paolo Arosio
Jun 2, 2021·Nature Communications·Mohammad Peydayesh, Raffaele Mezzenga
Jun 25, 2021·Chemical Science·Andreas M KüffnerPaolo Arosio
Jul 2, 2021·Biomacromolecules·Marta Díaz-CaballeroSalvador Ventura
Feb 23, 2020·ACS Synthetic Biology·Ellen H ReedDaniel A Hammer
Nov 3, 2021·Nature Communications·Andrea TestaPaola Laurino
Oct 20, 2021·Advanced Materials·Umberto Capasso PalmieroPaolo Arosio
Aug 18, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Miriam LinsenmeierPaolo Arosio

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