Multifunctional solvothermal carbon derived from alginate using 'water-in-deep eutectic solvents' for enhancing enzyme activity.

Chemical Communications : Chem Comm
Niketa YadavDibyendu Mondal

Abstract

Herein, the use of a "water-in-deep eutectic solvent (DES)" system has been shown as an alternative platform for the low temperature conversion of alginic acid (AA) to a multifunctional aliginate derived carbon (AAC) material with variable oxygen functionalities. Using the in-built oxygenated functionalities in AAC, cytochrome-c (Cyt-c) was immobilised on the surface of AACs and the peroxidase activity of the enzyme was studied. Remarkably, the enzymatic activity was enhanced up to 5.5-fold compared to the native protein without compromising the structural stability of Cyt-c. Altogether, the present study demonstrates a sustainable process for the preparation of AACs and shows their beneficial effect on the activity and stability of Cyt-c, and thus, their application as a protein-friendly material for biotechnological applications.

References

Jul 18, 2001·Journal of the American Chemical Society·R J ChenH Dai
May 28, 2003·European Biophysics Journal : EBJ·Silke OellerichPeter Hildebrandt
Dec 22, 2009·Chemical Society Reviews·Maria-Magdalena Titirici, Markus Antonietti
Apr 18, 2013·Angewandte Chemie·Pengfei ZhangYong Wang
Jan 1, 2012·Computational and Structural Biotechnology Journal·Shuang LiXiaoning Wang
Sep 19, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Maheshkumar Jaganathan, Aruna Dhathathreyan
Oct 11, 2014·Chemical Reviews·Emma L SmithKarl S Ryder
Oct 28, 2015·Essays in Biochemistry·Peter K Robinson
Oct 3, 2018·Green Chemistry : an International Journal and Green Chemistry Resource : GC·Meena BishtJ A P Coutinho
Oct 10, 2018·Chemical Society Reviews·Chunyan MaXiaoyan Ji
May 3, 2019·Applied Microbiology and Biotechnology·John M Woodley
Jan 4, 2020·Archives of Biochemistry and Biophysics·Santiago Oviedo-RoucoDaniel H Murgida

❮ Previous
Next ❯

Citations

Jul 11, 2021·Advanced Science·Alexander M CurreriEden E L Tanner

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensing
X-ray
circular dichroism

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.