Multifunctional TPP-PEG-biotin self-assembled nanoparticle drug delivery-based combination therapeutic approach for co-targeting of GRP78 and lysosome.

Journal of Nanobiotechnology
Baskaran PurushothamanJoon Myong Song

Abstract

In this study, a multifunctional tetraphenylporphyrin (TPP) conjugated polyethylene glycol with biotin (TPP-PEG-biotin) as a photo-dynamic therapy (PDT) material encapsulating a ruthenium complex 1 (Ru-1) was fabricated as self-assembled nanoparticle (Ru-1@TPP-PEG-biotin SAN) to co-target glucose-regulated protein 78 (GRP78) and the lysosome as a new anti-cancer therapeutic strategy. The MTT assay results reveals the enhanced anticancer activity of the Ru-1@TPP-PEG-biotin SANs due to the co-targeting of the GRP78 and lysosome. The Ru-1@TPP-PEG-biotin reduced level of GRP78 and lysosomal ceramide that contributed to the stability of the lysosomal membrane. The endoplasmic reticulum (ER) stress concomitant with the inhibition of GRP78 was clearly monitored by the phosphorylation of protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), and inositol-requiring enzyme 1 α (IRE1α) kinases to indicate the activation of the unfolded protein response (UPR) signaling using immunofluorescence assay. On the other hand, the degradation of the lysosome was observed through PDT action by the Ru-1@TPP-PEG-biotin SAN treatment. This was confirmed by the co-localization assay showing the disappearance of cathepsin D and lysosomal-assoc...Continue Reading

References

Jul 29, 2006·Autophagy·Ana Maria CuervoAlexei Terman
Jan 15, 2008·Cell·Beth Levine, Guido Kroemer
Dec 2, 2009·Methods in Molecular Biology·Jean-Pierre Gillet, Michael M Gottesman
Jun 24, 2010·ACS Nano·Marek GrzelczakLuis M Liz-Marzán
Jan 25, 2011·Current Opinion in Genetics & Development·Robin Mathew, Eileen White
Apr 24, 2013·Nature Reviews. Molecular Cell Biology·Carmine SettembreAndrea Ballabio
Jul 3, 2013·Nature Cell Biology·Patricia BoyaPatrice Codogno
Feb 1, 2015·Apoptosis : an International Journal on Programmed Cell Death·Carmen Garcia-RuizJosé C Fernández-Checa
Feb 13, 2015·Chemical Communications : Chem Comm·Xin DengBin He
Apr 9, 2015·Journal of Peptide Science : an Official Publication of the European Peptide Society·Chenhong WangNing Zhou
Oct 13, 2015·Advanced Drug Delivery Reviews·Jung Soo SukLaura M Ensign
May 14, 2016·ACS Applied Materials & Interfaces·Ruiyun ZhangXuehai Yan
Aug 4, 2016·Angewandte Chemie·Yonghyun LeeSangyong Jon
Aug 16, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Maja NešićMarijana Petković
Apr 15, 2017·Oncotarget·Reza Bayat MokhtariHerman Yeger
May 17, 2017·Advanced Healthcare Materials·Yunlu DaiFrank Caruso
Jun 10, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hohyeon LeeHyuncheol Kim
Jul 27, 2018·European Journal of Medicinal Chemistry·Baskaran PurushothamanJoon Myong Song
Sep 21, 2018·Journal of Nanobiotechnology·Jayanta Kumar PatraHan-Seung Shin
Sep 28, 2018·Advanced Materials·Wei-Hai ChenXian-Zheng Zhang
Oct 24, 2018·International Journal of Molecular Sciences·Sangiliyandi GurunathanJin-Hoi Kim
Dec 15, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wan-Ru ZhuangYu-Kyoung Oh
Dec 24, 2018·European Journal of Medicinal Chemistry·Chunlin ZhuangZhenyuan Miao
Sep 4, 2019·Lancet·Cindy H ChauWilliam D Figg
Jan 7, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Baskaran PurushothamanJoon Myong Song

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Methods Mentioned

BETA
dynamic light scattering
fluorescence
fluorescence microscopy
PCA
electrophoretic light scattering
dynamic

Software Mentioned

[UNK]

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