Multifunctionalization of Cells with a Self-Assembling Molecule to Enhance Cell Engraftment

ACS Chemical Biology
Ippei TakashimaMotonari Uesugi

Abstract

Cell-based therapy is a promising approach to restoring lost functions to compromised organs. However, the issue of inefficient cell engraftment remains to be resolved. Herein, we take a chemical approach to facilitate cell engraftment by using self-assembling molecules which modify two cellular traits: cell survival and invasiveness. In this system, the self-assembling molecule induces syndecan-4 clusters on the cellular surface, leading to enhanced cell viability. Further integration with Halo-tag technology provided this self-assembly structure with matrix metalloproteinase-2 to functionalize cells with cell-invasion activity. In vivo experiments showed that the pretreated cells were able to survive injection and then penetrate and engraft into the host tissue, demonstrating that the system enhances cell engraftment. Therefore, cell-surface modification via an alliance between self-assembling molecules and ligation technologies may prove to be a promising method for cell engraftment.

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Citations

Sep 27, 2020·Angewandte Chemie·Shuyu JinMotonari Uesugi
Jan 15, 2021·Chemical Communications : Chem Comm·Hayase HakariyaMotonari Uesugi
Oct 26, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yukiya TakayamaMakiya Nishikawa
Apr 9, 2021·ACS Applied Materials & Interfaces·Dingze MangYe Zhang
Aug 3, 2021·Biological & Pharmaceutical Bulletin·Kosuke Kusamori
Apr 9, 2020·ACS Applied Materials & Interfaces·Sona Rani RoyYe Zhang

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