Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase

Scientific Reports
Alex PudneyOlga V Sayanova

Abstract

There is an urgent requirement for sustainable sources of food and feed due to world population growth. Aquaculture relies heavily on the fish meal and fish oils derived from capture fisheries, challenging sustainability of the production system. Furthermore, substitution of fish oil with vegetable oil and fish meal with plant seed meals in aquaculture feeds reduces the levels of valuable omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, and lowers the nutritional value due to the presence of phytate. Addition of exogenous phytase to fish feed is beneficial for enhancing animal health and reducing phosphorus pollution. We have engineered the marine diatom Phaeodactylum tricornutum, accumulating high levels of EPA and DHA together with recombinant proteins: the fungal Aspergillus niger PhyA or the bacterial Escherichia coli AppA phytases. The removal of the N-terminal signal peptide further increased phytase activity. Strains engineered with fcpA and CIP1 promoters showed the highest level of phytase activity. The best engineered strain achieved up to 40,000 phytase activity units (FTU) per gram of soluble protein, thus demonstrating the feasibility of development of m...Continue Reading

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Citations

Jun 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana AlvesMadalena Pinto
Aug 23, 2020·Antioxidants·Francesco ManfellottoMaria Immacolata Ferrante
Feb 10, 2020·International Journal of Biological Macromolecules·Muhammad Bilal, Hafiz M N Iqbal
Aug 29, 2021·Plants·Daniela CastigliaSergio Esposito
Aug 29, 2021·World Journal of Microbiology & Biotechnology·Imke de Grahl, Sigrun Reumann

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Datasets Mentioned

BETA
AAN28334
AAR08366

Methods Mentioned

BETA
transgenic
PCR
phytase assay

Software Mentioned

GenScript
SignalP
GenStat

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