Multilayer Coating of Tetrandrine-loaded PLGA nanoparticles: Effect of surface charges on cellular uptake rate and drug release profile

Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Rui MengChen Shi

Abstract

The effect of surface charges on the cellular uptake rate and drug release profile of tetrandrine-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (TPNs) was studied. Stabilizer-free nanoprecipitation method was used in this study for the synthesis of TPNs. A typical layer-by-layer approach was applied for multi-coating particles' surface with use of poly(styrene sulfonate) sodium salt (PSS) as anionic layer and poly(allylamine hydrochloride) (PAH) as cationic layer. The modified TPNs were characterized by different physicochemical techniques such as Zeta sizer, scanning electron microscopy and transmission electron microscopy. The drug loading efficiency, release profile and cellular uptake rate were evaluated by high performance liquid chromatography and confocal laser scanning microscopy, respectively. The resultant PSS/PAH/PSS/PAH/TPNs (4 layers) exhibited spherical-shaped morphology with the average size of 160.3±5.165 nm and zeta potential of-57.8 mV. The encapsulation efficiency and drug loading efficiency were 57.88% and 1.73%, respectively. Multi-layer coating of polymeric materials with different charges on particles' surface could dramatically influence the drug release profile of TPNs (4 layers vs. 3 layers...Continue Reading

References

Mar 20, 2004·Science·Theresa M Allen, Pieter R Cullis
Sep 10, 2005·American Journal of Respiratory and Critical Care Medicine·Svetlana GelperinaLeonid Heifets
Nov 18, 2006·International Journal of Pharmaceutics·Jörg Kreuter
Mar 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kwangjae ChoDong M Shin
Mar 21, 2009·Bioorganic & Medicinal Chemistry·Amir H Faraji, Peter Wipf
Jun 6, 2009·Cellular and Molecular Life Sciences : CMLS·Hervé Hillaireau, Patrick Couvreur
May 25, 2010·International Journal of Pharmaceutics·Hagar I Labouta, Marc Schneider
Oct 23, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Nico ReumMarc Schneider
Apr 9, 2013·Journal of Natural Medicines·Tao ChenYancui Chen
Feb 20, 2014·International Journal of Clinical Pharmacology and Therapeutics·Youran ChenXiaoyan Zhao
Dec 3, 2014·Journal of the Chinese Medical Association : JCMA·Chin-Tsung TingTung-Hu Tsai
Dec 17, 2014·International Journal of Pharmaceutics·Chen ShiMarc Schneider

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Citations

Oct 24, 2017·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Qing GuoShun-Qing Xu
Aug 7, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Dan Chau Thuy NguyenLaurence Fitzhenry

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