Multilayered distribution of peptidoglycan in the periplasmic space of Escherichia coli.

Journal of General Microbiology
M LeducJ Van Heijenoort

Abstract

When a staining technique using phosphotungstic acid (PTA) in 10% (w/v) chromic acid was applied to cells of Escherichia coli, the periplasmic space was seen as a dark 15-nm-thick layer of uniform appearance and constant width. Our observations are consistent with peptidoglycan being the main material stained. Isolated sacculi as well as purified peptidoglycan (protein free) were also stained by the same procedure, the thickness of the peptidoglycan being 8.8 +/- 1.8 and 6.6 +/- 1.5 nm, respectively. The increased thickness of the PTA-stained layer in stationary phase cells correlated well with the increased thickness of isolated sacculi or purified peptidoglycan and with the increased amount of peptidoglycan in such cells. Thickness measurements on isolated peptidoglycan were compatible with a two to three layer structure for material from exponential phase cells and with a four to five layer structure for that from stationary phase cells. Furthermore, the results indicated an uneven distribution of peptidoglycan material in the periplasmic space, the peptidoglycan spanning the space from the inner to the outer membrane.

Citations

Jan 1, 1995·Biotechnology Advances·A P Middelberg
Jan 1, 1992·Electron Microscopy Reviews·L L Graham
Jan 1, 1990·Research in Microbiology·J V Höltje, B Glauner
Jul 3, 2013·Journal of Bacteriology·Elitza I TochevaGrant J Jensen
Feb 15, 2000·Journal of Bacteriology·E Hoiczyk, A Hansel
May 20, 2003·Journal of Bacteriology·Boris A DmitrievStefan Ehlers
Sep 3, 2004·Journal of Bacteriology·Waldemar Vollmer, Joachim-Volker Höltje
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Kelly C Rice, Kenneth W Bayles
Jan 16, 2008·FEMS Microbiology Reviews·Waldemar VollmerMiguel A de Pedro
Mar 1, 1990·FEMS Microbiology Reviews·U Wanner, T Egli
Dec 19, 2009·PLoS Pathogens·Mathilde GendrinBruno Lemaitre
Nov 25, 2006·Critical Reviews in Microbiology·Ann M J Diels, Chris W Michiels
Jan 21, 2021·EcoSal Plus·Shambhavi GardeManjula Reddy

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