Multilayered semiconducting polymer nanoparticles with enhanced NIR fluorescence for molecular imaging in cells, zebrafish and mice

Chemical Science
Houjuan ZhuKanyi Pu

Abstract

Although organic semiconducting polymer nanoparticles (SPNs) have emerged as an important category of optical imaging agents, their application in molecular imaging is still in its infancy and faces many challenges. We herein report a straightforward one-pot synthetic approach to construct multilayered near-infrared (NIR) fluorescent SPNs with enhanced fluorescence and optimized biodistribution for in vivo molecular imaging. In addition to the SP core, the multilayered SPNs have a middle silica protection layer and an outer poly(ethylene glycol) (PEG) corona, which play crucial roles in enhancing the NIR fluorescence by up to ∼100 fold and reducing nonspecific interactions, respectively. Their proof-of-concept imaging applications are demonstrated in cells, zebrafish and living mice. The multilayered nanoarchitecture not only permits in vivo lymph node tracking with an ultrahigh signal-to-noise ratio (∼85), but also allows for more sensitive in vivo imaging of tumors with a fluorescence intensity ratio of tumor to liver that is ∼8-fold higher compared to that of the counterpart silica SPN. Thus, this study provides a simple yet effective nanoengineering approach to facilitate the application of SPNs in molecular imaging.

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Citations

Sep 22, 2017·Current Medicinal Chemistry·Houjuan ZhuKanyi Pu
Jan 16, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Thais Fedatto AbelhaLea Ann Dailey
Aug 7, 2018·Chemical Communications : Chem Comm·Hannah R ShanksBrian R Saunders
Dec 6, 2019·Frontiers in Bioengineering and Biotechnology·Yu YangXinxin Li
Sep 28, 2017·Methods and Applications in Fluorescence·Enrico RampazzoNelsi Zaccheroni
Apr 13, 2017·Macromolecular Rapid Communications·Dong CuiKanyi Pu
Mar 17, 2019·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Yufan ZhangLiqin Xiong
Jun 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dong CuiKanyi Pu
Nov 13, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jun DengJun Zhao
Dec 22, 2016·Bioconjugate Chemistry·Qingqing Miao, Kanyi Pu
Nov 7, 2019·ACS Applied Materials & Interfaces·Guoliang YangWeian Zhang
Dec 12, 2020·ACS Applied Materials & Interfaces·Fang WangTonglei Cheng
Oct 3, 2018·Analytical Chemistry·Chun-Ting KuoDaniel T Chiu
Nov 21, 2019·ACS Applied Materials & Interfaces·Paul Robert NeumannLea Ann Dailey

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Methods Mentioned

BETA
nanoprecipitation
fluorescence imaging
light scattering
Fluorescence
surgical resection

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