Multilayered thin films that sustain the release of functional DNA under physiological conditions

Langmuir : the ACS Journal of Surfaces and Colloids
Jingtao ZhangDavid M Lynn

Abstract

The development of thin films and coatings that control the release of DNA from the surfaces of materials could have a significant impact on localized approaches to gene therapy. Here, we report multilayered polyelectrolyte assemblies that sustain the release of functional plasmid DNA from the surfaces of model substrates under physiological conditions. Multilayered assemblies consisting of alternating layers of plasmid DNA encoding for enhanced green fluorescent protein (EGFP) and a synthetic degradable polyamine were deposited on planar silicon and quartz substrates using a layer-by-layer fabrication process. Film growth was monitored by ellipsometry and UV spectrophotometry and correlated linearly with the number of polymer and plasmid layers deposited. In general, the thickness of deposited layers was found to be a function of both the pH and the ionic strength of the polyelectrolyte solutions used. Films up to 100 nm thick were investigated in this study. These assemblies erode gradually upon incubation in phosphate-buffered saline at 37 degrees C, as determined by ellipsometry and UV spectrophotometry, and sustain the release of incorporated plasmid into the incubation medium for a period of up to 30 h. Characterization o...Continue Reading

References

Jun 29, 1999·Nature Biotechnology·W M Saltzman
Nov 4, 2000·Nature Biotechnology·B D KlugherzR J Levy
Jul 18, 2001·Journal of the American Chemical Society·S T DubasJ B Schlenoff
Nov 17, 2001·Biomacromolecules·C Schüler, F Caruso
Apr 12, 2002·Journal of Biomedical Materials Research·Petra Tryoen-TóthJoëlle Ogier
May 16, 2002·Bioconjugate Chemistry·Tatiana Segura, Lonnie D Shea
Jul 18, 2002·Nature Reviews. Drug Discovery·W Mark Saltzman, William L Olbricht
Nov 12, 2002·Biomacromolecules·L RichertC Picart
Nov 21, 2002·Journal of the American Chemical Society·Eduardo VázquezDavid M Lynn
Jan 14, 2003·Biomacromolecules·Jonas D MendelsohnMichael F Rubner
Mar 18, 2003·Angewandte Chemie·Takeshi SerizawaMitsuru Akashi
Jun 19, 2003·Journal of the American Chemical Society·Benjamin ThierryMaryam Tabrizian
Nov 11, 2003·Biomacromolecules·Benjamin ThierryMaryam Tabrizian
Mar 18, 2004·Journal of the American Chemical Society·Dmitry G ShchukinYuri M Lvov

❮ Previous
Next ❯

Citations

Nov 5, 2013·Journal of Colloid and Interface Science·Cédric C Buron, Claudine Filiâtre
Feb 7, 2013·ACS Applied Materials & Interfaces·Han ShihChien-Chi Lin
Jan 4, 2013·ACS Applied Materials & Interfaces·Christina A Holmes, Maryam Tabrizian
Apr 20, 2013·Biomacromolecules·Eric M SaurerDavid M Lynn
Oct 13, 2007·Biomacromolecules·Hironobu TakahashiDavid W Grainger
Mar 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Javeed Shaikh MohammedMichael J McShane
May 18, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Ryan M FlessnerDavid M Lynn
Mar 29, 2007·Chemical Communications : Chem Comm·Yang ZhongDonald T Haynie
Jul 7, 2010·Chemical Communications : Chem Comm·Mahmoud SolimanCameron Alexander
Feb 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kris C WoodPaula T Hammond
Oct 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Maria DimitrovaThomas F Baumert
Sep 1, 2009·Future Medicinal Chemistry·Steven J P McInnes, Nicolas H Voelcker
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kris C WoodPaula T Hammond
Jun 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·N JesselJ-C Voegel
Jan 1, 2010·Soft Matter·Daniel A Bernards, Tejal A Desai
Jul 19, 2012·Chemical Communications : Chem Comm·Qingmin JiKatsuhiko Ariga
Feb 17, 2009·Expert Opinion on Biological Therapy·Katsuhiko ArigaGuoping Chen
Mar 6, 2010·Expert Opinion on Drug Delivery·Ron B ShmueliJordan J Green
Jun 2, 2009·Expert Opinion on Drug Delivery·Sania MansouriMaryam Tabrizian
Jun 13, 2009·Expert Opinion on Drug Delivery·Bruno G De GeestHelmuth Möhwald
Dec 25, 2012·Colloids and Surfaces. B, Biointerfaces·Aya Mizutani AkimotoMizuo Maeda
Jun 21, 2011·Advanced Drug Delivery Reviews·Svetlana Pavlukhina, Svetlana Sukhishvili
Jun 24, 2015·Colloids and Surfaces. B, Biointerfaces·Wei-Wen Hu, Yan-Rong Zheng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.