Multilevel samplers as microcosms to assess microbial response to biostimulation

Ground Water
B R BaldwinDavid C White

Abstract

Passive multilevel samplers (MLS) containing a solid matrix for microbial colonization were used as in situ microcosms in conjunction with a push-pull biostimulation experiment designed to promote biological U(VI) and Tc(VII) reduction. MLS were deployed at 24 elevations in the injection well and two downgradient wells to investigate the spatial variability in microbial community composition and growth prior to and following biostimulation. The microbial community was characterized by real-time quantitative polymerase chain reaction (Q-PCR) quantification of bacteria, NO(3)(-)-reducing bacteria (nirS and nirK), delta-proteobacteria, Geobacter sp., and methanogens (mcrA). Pretest cell densities were low overall but varied substantially with significantly greater bacterial populations detected at circumneutral pH (t-test, alpha= 0.05), suggesting carbon substrate and low pH limitations of microbial activity. Although pretest cell densities were low, denitrifying bacteria were dominant members of the microbial community. Biostimulation with an ethanol-amended ground water resulted in concurrent NO(3)(-) and Tc(VII) reduction, followed by U(VI) reduction. Q-PCR analysis of MLS revealed significant (1 to 2 orders of magnitude, Mann-...Continue Reading

References

Feb 1, 1995·Applied and Environmental Microbiology·A OgramJ K Fredrickson
Apr 18, 1998·Applied and Environmental Microbiology·R A ThomasL E Macaskie
Jul 2, 1999·Applied and Environmental Microbiology·J N Rooney-VargaD R Lovley
Oct 31, 2000·Applied and Environmental Microbiology·M T SuzukiE F DeLong
Jun 27, 2001·Applied and Environmental Microbiology·Y J ChangD C White
Sep 26, 2001·Applied and Environmental Microbiology·W F RölingH W van Verseveld
Mar 28, 2002·Applied and Environmental Microbiology·Jutta KleikemperJosef Zeyer
Mar 7, 2003·Applied and Environmental Microbiology·Dhruva BhattacharyaBanwari Lal
Jun 6, 2003·Applied and Environmental Microbiology·Brett R BaldwinLoring Nies
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jonathan D Van HammeOwen P Ward
Jan 31, 2004·Environmental Science & Technology·J D IstokD C White
Mar 3, 2004·Microbial Ecology·A D PeacockD C White
Apr 21, 2004·Environmental Toxicology and Chemistry·Richard DobsonJosef Zeyer
Oct 6, 2005·Applied and Environmental Microbiology·Helen A VrionisDerek R Lovley
May 1, 1992·Microbial Ecology·H J Albrechtsen, A Winding
Dec 1, 1991·Microbial Ecology·T C HazenC B Fliermans

❮ Previous
Next ❯

Citations

Jan 6, 2011·Ground Water·Tara A KneeshawErik W Smith
Oct 16, 2015·Environmental Science & Technology·Yuanyuan LiuCharles T Resch
Jun 5, 2015·Frontiers in Microbiology·Grace PoldKristen M DeAngelis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.