PMID: 9450918Feb 5, 1998Paper

Multilineage engraftment in NOD/LtSz-scid/scid mice from mobilized human CD34+ peripheral blood progenitor cells

Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation
Christopher J HoganGordon Keller

Abstract

Peripheral blood progenitor cells (PBPCs) are now the most widely used source for hematopoietic support of patients in the autologous transplant setting and are increasingly being used for allogeneic transplantation. A reliable model to characterize the in vivo potential of various PBPC subpopulations could be valuable as a preclinical assay to predict the hematopoietic performance in humans of these populations and of products resulting from their manipulations ex vivo. We have used immunocompromised nonobese diabetic/LtSz-scid/scid (NOD/SCID) mice to engraft human CD34+ PBPCs and to study the repopulation characteristics of this progenitor cell fraction. Following myeloablation, intravenous infusion of CD34+ cells consistently produced engraftment and development in mice. Multilineage development occurred in all mice with CD34+ cells, erythroid precursors, and the most immature populations of myeloid cells and B lymphocytes restricted to the mouse bone marrow (BM). More mature populations of myeloid cells and B lymphocytes were peripheralized to the spleen and blood of the animals. This finding suggests that human hematopoiesis in the mice may recapitulate hematopoietic recovery in humans. The provision of human growth factor...Continue Reading

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.