Multilineage hematopoietic engraftment after allogeneic peripheral blood stem cell transplantation without conditioning in SCID patients

Bone Marrow Transplantation
B BieloraiA Toren

Abstract

Successful stem cell transplantation for patients with severe combined immunodeficiency (SCID) from matched family donors without conditioning results in engraftment of T lymphocytes. B lymphocytes engraft in only 50% of the cases, while myelopoiesis and erythropoiesis remain of host origin. Full hematopoietic engraftment was reported in one case after bone marrow transplantation without conditioning for a SCID patient. We studied three SCID patients who were transplanted with unmodified mobilized peripheral blood from HLA-identical family sex-mismatched members. They received megadoses of stem cells (18-23 x 10(6)CD34/kg). In contrast to the expected mixed chimerism that usually occurs in the absence of conditioning, we found in our patients 100% donor cell engraftment based on fluorescence in situ hybridization (FISH) and microsatellite techniques. Subset analysis of the engrafted cells using a multiparametric system enabling a combined analysis of morphology, immunophenotyping and FISH showed that both T and B lymphocytes and myeloid cells were of donor origin in two patients, while T lymphocytes and myeloid cells were of donor origin in the third. In the two cases with ABO incompatibility, erythroid engraftment was evidence...Continue Reading

References

Feb 18, 1999·The New England Journal of Medicine·R H BuckleyF E Ward

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Citations

Feb 2, 2010·Immunology and Allergy Clinics of North America·Christopher C Dvorak, Morton J Cowan
Sep 1, 2005·Clinics in Laboratory Medicine·Süreyya Savaşan, Esteban M Abella
Oct 8, 2005·Stem Cells·Taina JaatinenJukka Partanen
May 19, 2012·Pediatric Transplantation·Funda Erol CipeAydan Ikinciogullari
Feb 25, 2006·Xenotransplantation·Maddalena SonciniOrnella Parolini
Sep 23, 2008·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Christopher C DvorakMorton J Cowan
May 5, 2010·Oncology Nursing Forum·Melissa Baker
Aug 29, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Georg StussiJörg D Seebach
Oct 27, 2004·Bone Marrow Transplantation·P BaderT Klingebiel
Aug 11, 2005·Canadian Journal of Physiology and Pharmacology·Bruce A BunnellPhilip J Kadowitz
Jan 4, 2007·Pediatrics·Bertram H LubinWilliam T Shearer

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