Multilocus genotyping of Enterocytozoon bieneusi derived from nonhuman primates in southwest China

PloS One
Zhijun ZhongGuangneng Peng

Abstract

Enterocytozoon bieneusi has been increasingly reported in non-human primates (NHPs) in recent years, and this has garnered attention. However, reports of E. bieneusi infections in NHPs are limited worldwide. To appreciate the genetic diversity and assess the zoonotic potential during the transmission of human microsporidiosis, we examined a total of 369 fecal samples from NHPs and performed PCR amplification of the ITS gene of E. bieneusi. An infection rate of 12.5% (46/369) was detected in NHPs, with three known genotypes (D, PigEBITS7, and SC02) and a novel genotype (SCM01) characterized. Phylogenetic analysis indicated that all four genotypes in our study were classified as zoonotic group 1. Multilocus genotyping of positive E. bieneusi strains revealed that 36, 37, 30, and 29 specimens were successfully amplified and sequenced to generate 16, six, four, and five types of MS1, MS3, MS4, and MS7 loci, respectively. Twenty-four specimens were successfully amplified and sequenced at all four loci, forming 13 multilocus genotypes (MLGs). The occurrence of zoonotic genotypes suggests that zoonotic transmission of E. bieneusi between humans and NHPs has probably occurred and NHPs could be a source of human microspordiosis.

References

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Citations

May 2, 2019·Parasite : Journal De La Société Française De Parasitologie·Run LuoGuangneng Peng
Mar 14, 2018·Parasites & Vectors·Chuanxiang TangLihua Xiao
Sep 3, 2019·Frontiers in Microbiology·Aiyun ZhaoLongxian Zhang
Jun 11, 2021·Transboundary and Emerging Diseases·Md Robiul KarimLongxian Zhang

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Datasets Mentioned

BETA
KX905208-KX905211

Methods Mentioned

BETA
genotyping
PCR
electrophoresis

Software Mentioned

SPSS
Mega
BLAST
MLST
ClustalX

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