MultiLocus Sequence Analysis- and Amplified Fragment Length Polymorphism-based characterization of xanthomonads associated with bacterial spot of tomato and pepper and their relatedness to Xanthomonas species

Systematic and Applied Microbiology
A A HamzaO Pruvost

Abstract

MultiLocus Sequence Analysis (MLSA) and Amplified Fragment Length Polymorphism (AFLP) were used to measure the genetic relatedness of a comprehensive collection of xanthomonads pathogenic to solaneous hosts to Xanthomonas species. The MLSA scheme was based on partial sequences of four housekeeping genes (atpD, dnaK, efp and gyrB). Globally, MLSA data unambiguously identified strains causing bacterial spot of tomato and pepper at the species level and was consistent with AFLP data. Genetic distances derived from both techniques showed a close relatedness of (i) X. euvesicatoria, X. perforans and X. alfalfae and (ii) X. gardneri and X. cynarae. Maximum likelihood tree topologies derived from each gene portion and the concatenated data set for species in the X. campestris 16S rRNA core (i.e. the species cluster comprising all strains causing bacterial spot of tomato and pepper) were not congruent, consistent with the detection of several putative recombination events in our data sets by several recombination search algorithms. One recombinant region in atpD was identified in most strains of X. euvesicatoria including the type strain.

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Citations

May 14, 2014·Annual Review of Phytopathology·Boris A VinatzerChristopher R Clarke
Dec 8, 2015·Frontiers in Microbiology·Bukola R Aremu, Olubukola O Babalola
Mar 19, 2014·Journal of Microbiological Methods·Sophie CesbronCharles Manceau
Dec 26, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Lijuan YangQiangming Sun
Oct 11, 2016·The Plant Pathology Journal·Min-Seong KyeonJae-Soon Cha
Feb 3, 2018·Molecular Medicine Reports·Jianhui ZhangShun Zhang
Jan 10, 2018·Frontiers in Plant Science·Noura YahiaouiStéphane Poussier
Apr 17, 2019·Journal of Applied Genetics·Gaia BertaniCamilla Lazzi
Sep 3, 2021·Molecular Plant Pathology·Ebrahim OsdaghiGary E Vallad

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