Multilocus sequence and microsatellite identification of intra-specific hybrids and ancestor-like donors among natural Ethiopian isolates of Leishmania donovani

International Journal for Parasitology
Tesfaye GelanewMatthew Yeo

Abstract

Protozoan parasites of the genus Leishmania (Kinetoplastida: Trypanosomatidae) cause widespread and devastating human diseases. Visceral leishmaniasis is endemic in Ethiopia where it has also been responsible for fatal epidemics. It is postulated that genetic exchange in Leishmania has implications for heterosis (hybrid vigour), spread of virulent strains, resistance to chemotherapeutics, and exploitation of different hosts and vectors. Here we analyse 11 natural Ethiopian Leishmania donovani isolates consisting of four putative hybrids, seven parent-like isolates and over 90 derived biological clones. We apply a novel combination of high resolution multilocus microsatellite typing (five loci) and multilocus sequence typing (four loci) that together distinguish parent-like and hybrid L. donovani strains. Results indicate that the four isolates (and their associated biological clones) are genetic hybrids, not the results of mixed infections, each possessing heterozygous markers consistent with inheritance of divergent alleles from genetically distinct Ethiopian L. donovani lineages. The allelic profiles of the putative hybrids may have arisen from a single hybridisation event followed by inbreeding or gene conversion, or alterna...Continue Reading

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Citations

Feb 3, 2016·Evolutionary Applications·Joanne P WebsterAndy Fenton
Jul 19, 2015·Parasites & Vectors·E GadisaAlvar Jorge
Feb 6, 2017·Molecular Aspects of Medicine·Mohammad AkhoundiDenis Sereno
Apr 21, 2020·PLoS Neglected Tropical Diseases·James A CottonMatthew Yeo
Oct 4, 2019·Acta Tropica·Juan Jose LauthierMasataka Korenaga
Dec 29, 2020·Frontiers in Cellular and Infection Microbiology·Roman Telittchenko, Albert Descoteaux
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May 24, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sofia El KacemMeryem Lemrani

Methods Mentioned

BETA
electrophoresis
transgenic
PCR
two
four hybrid
five hybrid
fluorescence activated cell sorting
FACS
two hybrids

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