Multilocus sequence typing and virulence gene profiles associated with Escherichia coli from human and animal sources

Foodborne Pathogens and Disease
Amee R MangesPatrick Boerlin

Abstract

We investigated whether specific sequence types, and their shared virulence gene profiles, may be associated with both human and food animal reservoirs. A total of 600 Escherichia coli isolates were assembled from human (n=265) and food-animal (n=335) sources from overlapping geographic areas and time periods (2005-2010) in Canada. The entire collection was subjected to multilocus sequence typing and a subset of 286 E. coli isolates was subjected to an E. coli-specific virulence gene microarray. The most common sequence type (ST) was E. coli ST10, which was present in all human and food-animal sources, followed by ST69, ST73, ST95, ST117, and ST131. A core group of virulence genes was associated with all 10 common STs including artJ, ycfZ, csgA, csgE, fimA, fimH, gad, hlyE, ibeB, mviM, mviN, and ompA. STs 73, 92, and 95 exhibited the largest number of virulence genes, and all were exclusively identified from human infections. ST117 was found in both human and food-animal sources and shared virulence genes common in extraintestinal pathogenic E. coli lineages. Select groups of E. coli may be found in both human and food-animal reservoirs.

References

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Citations

Apr 15, 2016·The Journal of Antimicrobial Chemotherapy·Shu-Chen KuoTsai-Ling Lauderdale
May 21, 2016·Frontiers in Microbiology·Pina M FratamicoPeter Feng
Oct 19, 2016·Avian Pathology : Journal of the W.V.P.A·C A BorgesF A Ávila
Sep 13, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F El GarchX Bertrand
Jan 8, 2018·Microbial Genomics·Cameron J ReidSteven P Djordjevic
Dec 8, 2019·Journal of Clinical Medicine·Ángel Rodríguez-VillodresYounes Smani
Jun 14, 2019·Clinical Microbiology Reviews·Amee R MangesJohann D D Pitout
Feb 14, 2018·Journal of Clinical Microbiology·Reina YamajiLee W Riley
Jan 16, 2020·International Journal of Environmental Research and Public Health·Ewa BokKatarzyna Baldy-Chudzik
Aug 11, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Bente Olesen
Feb 18, 2020·Frontiers in Microbiology·Cameron J ReidSteven P Djordjevic
Dec 1, 2020·Microbial Genomics·Ebenezer Foster-NyarkoMark John Pallen
Feb 4, 2021·International Journal of Molecular Sciences·Marta KsiążczykGabriela Bugla-Płoskońska
Dec 11, 2020·BMC Veterinary Research·Anat Shnaiderman-TorbanAmir Steinman
Jan 2, 2021·Microbiology Spectrum·Lee W Riley
May 25, 2021·Frontiers in Public Health·Silvia Adriana Mayer LentzAndreza Francisco Martins

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Methods Mentioned

BETA
MDS

Software Mentioned

R
ScanArray Express
NMDS

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