Multimarker Analysis of Circulating Tumor Cells in Peripheral Blood of Metastatic Breast Cancer Patients: A Step Forward in Personalized Medicine.

Breast Care
Andreia de AlbuquerqueNikos Fersis

Abstract

AIM: To develop an immunomagnetic assay for the isolation of circulating tumor cells (CTCs) followed by the analysis of a multimarker panel, which will enable the characterization of these malignant cells with high accuracy. PATIENTS AND METHODS: Peripheral blood (PB) was collected from 32 metastatic breast cancer patients and 42 negative controls. The antibodies BM7 and VU1D9 were used for immunomagnetic tumor cell enrichment. A real-time reverse transcription-polymerase chain reaction (RT-PCR) approach for the markers KRT19, SCGB2A2, MUC1, EPCAM, BIRC5 and ERBB2 was used for CTC detection and characterization. RESULTS: THE POSITIVITY RATES FOR EACH MARKER WERE AS FOLLOWS: 46.9% for KRT19, 25.0% for SCGB2A2, 28.1% for MUC1, 28.1% for EPCAM, 21.9% for BIRC5, and 15.6% for ERBB2. After the creation of individualized cutoffs, the sensitivity and specificity of the combined marker gene panel increased to 56.3% and 100%, respectively. Interestingly, 27.0% of the HER2-negative tumor patients showed ERBB2 mRNA-positive CTCs. CONCLUSIONS: The described technique can be used to measure CTCs with great accuracy. The use of a multimarker panel for the characterization of CTCs may provide real-time information and be of great value in ...Continue Reading

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Citations

Nov 14, 2012·Journal of Translational Medicine·Andreia de AlbuquerqueSepp Kaul
Mar 19, 2014·Cancers·Lori E Lowes, Alison L Allan
Aug 12, 2014·International Journal of Cancer. Journal International Du Cancer·Arutha KulasingheChamindie Punyadeera
Jan 11, 2013·Cell Adhesion & Migration·Teresa M Horm, Joyce A Schroeder
Mar 22, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P K GroverJ E Hardingham
Mar 8, 2018·Journal of Dental Research·T Nonaka, D T W Wong
Jul 11, 2018·Biotechnology and Bioengineering·Sultan KhetaniAmir Sanati Nezhad
May 26, 2018·The Analyst·Hyungseok ChoKi-Ho Han
Apr 30, 2013·Lab on a Chip·Sunil K AryaAbdur Rub Abdur Rahman
Mar 11, 2019·ACS Biomaterials Science & Engineering·Joel F LuskBarbara S Smith

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