PMID: 9005444Feb 1, 1996Paper

Multimer formation as a consequence of separate homodimerization domains: the human c-Jun leucine zipper is a transplantable dimerization module

Protein Engineering
L G RileyA S Weiss

Abstract

Human c-Jun and c-Fos leucine zipper domains were examined for their ability to serve as autonomous dimerization domains as part of a heterologous protein construct. Schistosoma japonicum glutathione S-transferase (GST) was fused to recombinant Jun leucine zipper (rJunLZ) and Fos leucine zipper (rFosLZ) domains. SDS-PAGE 'snapshot' analyses based on disulphide linkage of monomers demonstrated the ability of rJunLZ to function as a dimerization motif in a foreign protein environment. Steric hindrance prevented formation of rJunLZ-GST::rFosLZ-GST heterodimers whereas rJunLZ-GST::rFosLZ and rJunLZ:: rFosLZGST formed readily. Furthermore, rJunLZGST generated homodimers suggesting fusion protein heterodimers interact differently to homodimers. Gel filtration chromatography confirmed that GST is a dimer in solution and that attachment of a leucine zipper domain allows further interactions to take place. Sedimentation equilibrium analyses showed that GST is a stable dimer (K(a) > 10(6) M(-1)) with no higher multimeric forms. rFosLZ-GST weakly associates beyond a dimer (K(a) approximately 4 x 10(4) M(-1)) and rJunLZ-GST associates indefinitely (K(a) approximately 4 x 10(5) M(-1)) [corrected], consistent with an isodesmic model of assoc...Continue Reading

Citations

May 30, 1998·Protein Science : a Publication of the Protein Society·N LiuR M Thomas
Feb 26, 2013·The Journal of Biological Chemistry·Marylène VandevenneJoel P Mackay
Aug 4, 2015·Protein Science : a Publication of the Protein Society·Lorna Wilkinson-WhiteJacqueline M Matthews
Jun 17, 2008·Protein Science : a Publication of the Protein Society·Chu Kong LiewJoel P Mackay
Mar 1, 2012·Protein Science : a Publication of the Protein Society·Jesus Fernandez-Rodriguez, Thomas C Marlovits
May 6, 2009·Journal of Molecular Biology·Ingrid MacindoeMargaret Sunde
May 31, 2001·The Journal of Biological Chemistry·M T Drake, L M Traub

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