Multimerization of hepatitis delta antigen is a critical determinant of RNA binding specificity.

Journal of Virology
Brian C LinJohn L Casey

Abstract

Hepatitis delta virus (HDV) RNA forms an unbranched rod structure that is associated with hepatitis delta antigen (HDAg) in cells replicating HDV. Previous in vitro binding experiments using bacterially expressed HDAg showed that the formation of a minimal ribonucleoprotein complex requires an HDV unbranched rod RNA of at least about 300 nucleotides (nt) and suggested that HDAg binds the RNA as a multimer of fixed size. The present study specifically examines the role of HDAg multimerization in the formation of the HDV ribonucleoprotein complex (RNP). Disruption of HDAg multimerization by site-directed mutagenesis was found to profoundly alter the nature of RNP formation. Mutant HDAg proteins defective for multimerization exhibited neither the 300-nt RNA size requirement for binding nor specificity for the unbranched rod structure. The results unambiguously demonstrate that HDAg binds HDV RNA as a multimer and that the HDAg multimer is formed prior to binding the RNA. RNP formation was found to be temperature dependent, which is consistent with conformational changes occurring on binding. Finally, analysis of RNPs constructed with unbranched rod RNAs successively longer than the minimum length indicated that multimeric binding ...Continue Reading

References

Jun 1, 1990·The Journal of General Virology·T B MacnaughtonC J Burrell
Oct 9, 1986·Nature·A KosH Schellekens
Sep 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Rizzetto
Jan 1, 1993·Journal of Virology·J G Wang, S M Lemon
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·J E RozzelleS M Lemon
Jan 20, 1995·The Journal of Biological Chemistry·T Powers, H F Noller
May 1, 1993·Journal of Virology·D W Lazinski, J M Taylor
Jun 1, 1993·Journal of Virology·W S RyuJ Taylor
Sep 1, 1996·Journal of Virology·V V BichkoJ M Taylor
Aug 4, 1998·Structure·H J ZuccolaJ M Hogle
May 24, 2000·Journal of Virology·G MoraledaJ Taylor
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Sep 13, 2003·Journal of Virology·Cromwell T Cornillez-Ty, David W Lazinski
May 12, 2004·Protein Expression and Purification·Liuqing Yang, Zhi-Ren Liu
Jul 16, 2004·Journal of Virology·M A Mir, A T Panganiban
Aug 15, 2006·Current Topics in Microbiology and Immunology·T B Macnaughton, M M C Lai
Nov 23, 2007·Journal of Virology·Jinhong ChangJohn Taylor

❮ Previous
Next ❯

Citations

Apr 17, 2016·Journal of Hepatology·Camille Sureau, Francesco Negro
May 10, 2019·Nature Communications·Jimena Perez-VargasFrançois-Loïc Cosset
Oct 31, 2014·World Journal of Gastroenterology : WJG·Chi-Ruei Huang, Szecheng John Lo
Jan 23, 2020·Nature Communications·Natali Abeywickrama-SamarakoonPaul Dény
Jul 3, 2021·Viruses·Jimena Pérez-VargasNatalia Freitas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.