Multimerization of poly(rC) binding protein 2 is required for translation initiation mediated by a viral IRES

RNA
Kristin M BedardBert L Semler

Abstract

The cellular protein, poly(rC) binding protein 2 (PCBP2), is known to function in picornavirus cap-independent translation. We have further examined the RNA binding properties and protein-protein interactions of PCBP2 necessary for translation. We have studied its putative multimerization properties utilizing the yeast two-hybrid assay and in vitro biochemical methods, including glutathione S-transferase (GST) pull-down assays and gel filtration. Through genetic analysis, the multimerization domain has been localized to the second K-homologous (KH) RNA binding domain of the protein between amino acids 125 and 158. To examine the function of multimerization in poliovirus translation, we utilized the truncated protein, DeltaKH1-PCBP2, which is capable of multimer formation, but does not bind poliovirus stem-loop IV RNA (an interaction required for translation). Utilizing RNA binding and in vitro translation assays, this protein was shown to act as a dominant negative, suggesting that PCBP2 multimerization functions in poliovirus translation and RNA binding. Additionally, PCBP2 containing a deletion in the multimerization domain (DeltaKH2-PCBP2) was not able to bind poliovirus stem-loop IV RNA and could not rescue translation in e...Continue Reading

References

Jul 1, 1992·Journal of Virology·S L Dildine, B L Semler
May 1, 1995·Molecular and Cellular Biology·I M Weiss, S A Liebhaber
Mar 11, 1993·Nucleic Acids Research·H SiomiG Dreyfuss
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·L B BlynE Ehrenfeld
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·M Holcik, S A Liebhaber
Aug 1, 1997·Journal of Virology·L B BlynE Ehrenfeld
Sep 1, 1997·Molecular and Cellular Biology·B StefanovicD A Brenner
Apr 25, 2000·Journal of Molecular Biology·J H KimS K Jang
Jul 11, 2001·Genes & Development·C U Hellen, P Sarnow
May 11, 2002·RNA·Aleksandr V Makeyev, Stephen A Liebhaber

❮ Previous
Next ❯

Citations

Jun 22, 2007·Journal of Virology·Rushika PereraBert L Semler
May 15, 2009·Biochimica Et Biophysica Acta·Michael Niepmann
Jul 6, 2015·Methods : a Companion to Methods in Enzymology·Rosario Francisco-VelillaEncarnación Martínez-Salas
Dec 19, 2007·Biochemical and Biophysical Research Communications·Roland ZellMatthias Görlach
Oct 31, 2007·Biochemical and Biophysical Research Communications·Bo ZhangVerena Gauss-Müller
Nov 24, 2005·Virus Research·Richard E Lloyd
Jan 17, 2015·Virus Research·Aniko V Paul, Eckard Wimmer
Sep 4, 2010·Cellular Signalling·María Gabriela ThomasGraciela Lidia Boccaccio
Jan 13, 2010·Molecular Cell·J Ross Buchan, Roy Parker
Feb 24, 2009·Biochimica Et Biophysica Acta·Ken FujimuraMasayuki Murata
Jun 4, 2016·Nucleic Acids Research·Sawsan NapthineIan Brierley
Aug 16, 2013·Journal of Virology·Eduardo López-ManríquezAna Lorena Gutiérrez-Escolano
Dec 22, 2006·The EMBO Journal·Kristin M BedardBert L Semler
Mar 30, 2018·Frontiers in Microbiology·Michael NiepmannOliver Rossbach
Jun 24, 2017·Scientific Reports·Jian HanKian Leong Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.