Multimerization reactions of coxsackievirus proteins 2B, 2C and 2BC: a mammalian two-hybrid analysis

The Journal of General Virology
Arjan S de JongF J van Kuppeveld

Abstract

Recently, homomultimerization and heteromultimerization reactions of the poliovirus P2 region proteins were investigated using a yeast two-hybrid approach (Cuconati et al., Journal of Virology 72, 1297-1307, 1998). In this study, we investigated multimerization reactions of the 2B, 2C and 2BC proteins of the closely related coxsackie B3 virus (CBV3) using a mammalian two-hybrid system. This system allows the characterization of protein:protein interactions within a cellular environment that more closely mimics the native protein environment. Homomultimerization reactions were observed with the 2BC protein and, albeit weakly, with the 2B protein, but not with the 2C protein. To identify the determinants involved in the 2BC and 2B homomultimerization reactions, several mutants containing deletions or point mutations in the 2B region were tested. Disruption of the hydrophobic character of either the cationic amphipathic alpha-helix or the second hydrophobic domain of the 2B protein disturbed both the 2BC:2BC and the 2B:2B homomultimerization reactions. Disruption of either the cationic or the amphipathic character of the alpha-helix or deletion of the N-terminal 30 amino acids of the 2B protein, however, had no effect on the 2BC a...Continue Reading

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Citations

Nov 3, 2009·Virus Research·Kumiko IshikawaKoki Taniguchi
Feb 1, 2005·Trends in Microbiology·Frank J M van KuppeveldPeter H G M Willems
May 30, 2015·The Journal of General Virology·Claire Scott, Stephen Griffin
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Jun 13, 2009·The Journal of Biological Chemistry·Peter AdamsEllie Ehrenfeld
Jun 23, 2012·Virulence·Margot Carocci, Labib Bakkali-Kassimi
Sep 28, 2005·The Journal of General Virology·Roland ZellPeter Wutzler
Aug 17, 2002·The Journal of Biological Chemistry·Aitziber AgirreJosé L Nieva
Aug 13, 2003·The Journal of Biological Chemistry·Bettina HartliebStephan Becker
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May 1, 2021·Viruses·Anja GeislerHenry Fechner
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Aug 28, 2021·Microorganisms·Ines Cordeiro FilipeCaroline Tapparel
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