Abstract
Endotoxin-induced uveitis (EIU) in rodents is a model of acute Toll-like receptor 4 (TLR4)-mediated organ inflammation, and has been used to model human anterior uveitis, examine leukocyte trafficking and test novel anti-inflammatory therapeutics. Wider adoption has been limited by the requirement for manual, non-specific, cell-count scoring of histological sections from each eye as a measure of disease severity. Here, we describe a comprehensive and efficient technique that uses ocular dissection and multimodal tissue analysis. This allows matched disease scoring by multicolour flow cytometric analysis of the inflammatory infiltrate, protein analysis on ocular supernatants and qPCR on remnant tissues of the same eye. Dynamic changes in cell populations could be identified and mapped to chemokine and cytokine changes over the course of the model. To validate the technique, dose-responsive suppression of leukocyte infiltration by recombinant interleukin-10 was demonstrated, as well as selective suppression of the monocyte (CD11b+Ly6C+) infiltrate, in mice deficient for eitherCcl2orCcr2 Optical coherence tomography (OCT) was used for the first time in this model to allowin vivoimaging of infiltrating vitreous cells, and correlate...Continue Reading
References
Aug 7, 1980·Nature·J T RosenbaumP R Egbert
May 1, 1996·European Journal of Immunology·A D DickJ D Sedgwick
Nov 20, 1997·The Journal of Clinical Investigation·L BoringI F Charo
Jan 20, 1999·Immunology and Cell Biology·J R SmithK A Williams
Feb 8, 2006·Nature Immunology·Natalya V Serbina, Eric G Pamer
Jun 27, 2008·Gene Therapy·P TrittibachR R Ali
Dec 4, 2010·Journal of Neuroinflammation·William RaoulFlorian Sennlaub
Aug 19, 2011·PloS One·Mei ChenHeping Xu
Sep 22, 2011·Journal of Leukocyte Biology·Jordan J AllensworthHolly L Rosenzweig
Oct 21, 2011·Blood·Melanie J StablesDerek W Gilroy
Jan 4, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Shawn RoseHarris Perlman
May 1, 2012·PloS One·Ulrich F O LuhmannRobin R Ali
May 22, 2013·PloS One·Colin J ChuUlrich F O Luhmann
May 22, 2013·PloS One·Jun ChenRachel R Caspi
Jan 28, 2014·PloS One·Meredith J CraneJorge E Albina
Jul 10, 2014·Blood·Justine NewsonDerek W Gilroy
Aug 1, 2014·Journal of Leukocyte Biology·Dawn M E Bowdish
Nov 22, 2014·Investigative Ophthalmology & Visual Science·Laura Elizabeth DownieHolly Rose Chinnery
Jan 27, 2015·Journal of Autoimmunity·Samia YazidAndrew D Dick
Feb 5, 2015·Investigative Ophthalmology & Visual Science·Jennifer Rose-NussbaumerDavid Huang
Feb 24, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Cristhian J IldefonsoAlfred S Lewin
Citations
Nov 4, 2016·International Journal of Molecular Sciences·Inge Van HoveLieve Moons
Jan 24, 2017·Scientific Reports·Peter J GardnerJames W Bainbridge
Apr 5, 2018·Development·Enrico CristanteJames W B Bainbridge
Aug 21, 2019·Ocular Immunology and Inflammation·Haihan JiaoHolly R Chinnery
Jan 23, 2019·JCI Insight·Adam C MirandoNiranjan B Pandey
Jul 20, 2017·Journal of Neuroinflammation·Deborah A LipskiFrançois Willermain
May 31, 2018·Investigative Ophthalmology & Visual Science·Kathryn L PeppleRussell N Van Gelder
Jul 11, 2020·Scientific Reports·Sarah JohnKathryn L Pepple
Oct 3, 2018·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Haihan JiaoHolly R Chinnery
May 20, 2020·The British Journal of Ophthalmology·Ester CarreñoNicolas Alejandre
Jan 16, 2020·International Journal of Molecular Sciences·Jihae ParkJae Chan Kim
Aug 31, 2019·Biomedical Optics Express·Ruobing QianJoseph A Izatt
Jan 30, 2020·Frontiers in Immunology·Oliver H BellAndrew D Dick
Feb 12, 2021·Science Translational Medicine·Ying Kai ChanGeorge M Church
Feb 26, 2021·Investigative Ophthalmology & Visual Science·Gayathri TummalaKathryn L Pepple
Feb 23, 2021·Experimental Eye Research·Nu ChenHui Shao
Oct 15, 2020·ELife·Aby JosephJesse Schallek
Apr 4, 2021·International Journal of Molecular Sciences·Kenichi MakabeMasayo Takahashi
May 19, 2021·Translational Vision Science & Technology·Chulbul M AhmedAlfred S Lewin
May 18, 2021·Biomedical Optics Express·Ruobing QianJoseph A Izatt
Jul 6, 2021·Frontiers in Immunology·Lydia J BradleyLindsay B Nicholson
Aug 8, 2021·Microorganisms·Lin DuWai Kit Chu