Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance

Biotechnology for Biofuels
Mickaël HerbautGabriel Paës

Abstract

Biomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors. However, their relative importance remains challenging to evaluate. Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples. A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance. The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification). Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates. Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass. Lignin-related factors displayed highly negative coefficients for all bi...Continue Reading

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Citations

Apr 15, 2020·The New Phytologist·Joshua H CoomeySamuel P Hazen
Jan 11, 2020·Frontiers in Chemistry·Aya Zoghlami, Gabriel Paës
Sep 19, 2020·Biotechnology for Biofuels·Dennis WeidenerHolger Klose
Feb 5, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katja VasićMaja Leitgeb
May 25, 2021·International Journal of Biological Macromolecules·Ashvinder Kumar RanaVijay Kumar Thakur

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Methods Mentioned

BETA
nuclear magnetic resonance
scanning electron microscopy
NMR
Fluorescence
confocal microscopy

Software Mentioned

Eager
Leica Application Suite X
ImageJ
SigmaPlot

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