Multimodal chemical imaging of molecular messengers in emerging Pseudomonas aeruginosa bacterial communities

The Analyst
Nameera F BaigPaul W Bohn

Abstract

Two label-free molecular imaging techniques, confocal Raman microscopy (CRM) and secondary ion mass spectrometry (SIMS), are combined for in situ characterization of the spatiotemporal distributions of quinolone metabolites and signaling molecules in communities of the pathogenic bacterium Pseudomonas aeruginosa. Dramatic molecular differences are observed between planktonic and biofilm modes of growth for these bacteria. We observe patterned aggregation and a high abundance of N-oxide quinolines in early biofilms and swarm zones of P. aeruginosa, while the concentrations of these secreted components in planktonic cells and agar plate colonies are below CRM and SIMS detection limits. CRM, in conjunction with principal component analysis (PCA) is used to distinguish between the two co-localized isomeric analyte pairs 4-hydroxy-2-heptylquinoline-N-oxide (HQNO)/2-heptyl-3-hydroxyquinolone (PQS) and 4-hydroxy-2-nonylquinoline-N-oxide (NQNO)/2-nonyl-hydroxyquinolone (C9-PQS) based on differences in their vibrational fingerprints, illustrating how the technique can be used to guide tandem-MS and tandem-MS imaging analysis. Because N-oxide quinolines are ubiquitous and expressed early in biofilms, these analytes may be fundamentally i...Continue Reading

References

Jul 21, 2001·Lancet·P S Stewart, J W Costerton
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
Feb 25, 2003·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Renate PetryJürgen Popp
May 18, 2004·Journal of the American Society for Mass Spectrometry·François LépineLaurence G Rahme
Apr 12, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·U NeugebauerU Holzgrabe
Mar 1, 2006·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yu WangSihuan Wang
May 2, 2007·Mass Spectrometry Reviews·Liam A McDonnell, Ron M A Heeren
Aug 16, 2008·Molecular BioSystems·Jean-Frédéric Dubern, Stephen P Diggle
Sep 9, 2008·Trends in Microbiology·Natalie VerstraetenJan Michiels
Feb 7, 2009·Environmental Microbiology·T R de Kievit
Apr 3, 2010·Advances in Applied Microbiology·Wei E HuangSteven A Banwart
Aug 27, 2010·FEMS Microbiology Reviews·Stephan HeebMiguel Cámara
Dec 8, 2010·Future Microbiology·Niels HøibyThomas Bjarnsholt
Feb 23, 2013·The Analyst·Rachel MasyukoPaul W Bohn
Aug 19, 2014·Analytical Chemistry·Eric J LanniJonathan V Sweedler
Sep 4, 2014·Journal of the American Society for Mass Spectrometry·Eric J LanniJonathan V Sweedler
Dec 3, 2014·Current Opinion in Biotechnology·Yelena Ilin, Mary L Kraft

❮ Previous
Next ❯

Citations

Nov 4, 2016·Journal of Mass Spectrometry : JMS·Bin LiJonathan V Sweedler
Jun 16, 2017·ACS Chemical Biology·Erin GemperlineLingjun Li
Mar 29, 2018·The Journal of Biological Chemistry·Nydia Morales-SotoJoshua D Shrout
Mar 16, 2016·Journal of Bacteriology·Karen L VisickJean-Marc Ghigo
Jun 6, 2018·Frontiers in Cellular and Infection Microbiology·Gustavo BodelónIsabel Pastoriza-Santos
Apr 6, 2018·Clinical Microbiology Reviews·Maria MaganaGeorge P Tegos
Dec 24, 2018·Microbiology Insights·Nydia Morales-SotoJoshua D Shrout
Jan 1, 2017·Journal of Analysis and Testing·Jiayun Hu, Paul W Bohn
May 4, 2018·Analytical Chemistry·Bin LiJonathan V Sweedler
May 3, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Hyein DoPaul W Bohn
May 29, 2020·Analytical Chemistry·Junting ZhangPaulina D Rakowska
Dec 22, 2016·Accounts of Chemical Research·Sage J B DunhamJonathan V Sweedler
Apr 7, 2018·Analytical Chemistry·Sage J B DunhamJonathan V Sweedler
May 4, 2016·Journal of Proteome Research·Tong SiJonathan V Sweedler
Nov 25, 2020·ACS Nano·Matthias LorenzOlga S Ovchinnikova
Nov 14, 2018·ACS Nano·Alex BelianinovOlga S Ovchinnikova

❮ Previous
Next ❯

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.