Multimodal Functional Neuroimaging by Simultaneous BOLD fMRI and Fiber-Optic Calcium Recordings and Optogenetic Control

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Franziska AlbersCornelius Faber


Recent developments of optogenetic tools and fluorescence-based calcium recording techniques enable the manipulation and monitoring of neural circuits on a cellular level. Non-invasive imaging of brain networks, however, requires the application of methods such as blood oxygen level-dependent (BOLD) functional magnetic resonance imaging (fMRI), which is commonly used for functional neuroimaging. While BOLD fMRI provides brain-wide non-invasive reading of the hemodynamic response, it is only an indirect measure of neural activity. Direct observation of neural responses requires electrophysiological or optical methods. The latter can be combined with optogenetic control of neuronal circuits and are MRI compatible. Yet, simultaneous optical recordings are still limited to fiber-optic-based approaches. Here, we review the integration of optical recordings and optogenetic manipulation into fMRI experiments. As a practical example, we describe how BOLD fMRI in a 9.4-T small animal MR scanner can be combined with in vivo fiber-optic calcium recordings and optogenetic control in a multimodal setup. We present simultaneous BOLD fMRI and calcium recordings under optogenetic control in rat. We outline details about MR coil configuration, ...Continue Reading

Associated Proceedings Papers


Oct 15, 2019·Journal of Neuroimaging : Official Journal of the American Society of Neuroimaging·Andrew CroftsClaire L Gibson
Nov 21, 2018·Frontiers in Neuroscience·Patricia Pais-RoldánXin Yu
Mar 9, 2019·Nature Communications·Davide BoidoSerge Charpak
Nov 15, 2019·Scientific Reports·Nikoloz SirmpilatzeSusann Boretius


Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Jun 29, 2002·Science·Georg NagelPeter Hegemann
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christoph StosiekArthur Konnerth
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
Jul 12, 2005·Nature Neuroscience·Helmuth AdelsbergerArthur Konnerth
Aug 24, 2005·Nature Neuroscience·Edward S BoydenKarl Deisseroth
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jason N D KerrFritjof Helmchen
Apr 25, 2007·NMR in Biomedicine·F David DotyJohn P Staab
Nov 27, 2007·Journal of Molecular Biology·Christian BamannErnst Bamberg
Dec 13, 2007·Physiology·Werner Göbel, Fritjof Helmchen
Dec 28, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Viviana GradinaruKarl Deisseroth
Mar 18, 2008·Neuron·Liqun LuoKarel Svoboda
Dec 17, 2008·Nature Neuroscience·André BerndtKarl Deisseroth
Jul 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Katrin FeldbauerErnst Bamberg
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nathalie L RochefortArthur Konnerth
Oct 27, 2009·Current Opinion in Neurobiology·Benjamin F Grewe, Fritjof Helmchen
Jan 19, 2010·Nature Neuroscience·Lisa A GunaydinPeter Hegemann
Mar 23, 2010·Cell·Viviana GradinaruKarl Deisseroth
May 12, 2010·Magnetic Resonance Imaging·Kathleen A WilliamsShella D Keilholz
Dec 17, 2010·Journal of Neurophysiology·M DesaiEdward S Boyden
Apr 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Andre BerndtThomas G Oertner
Jun 23, 2011·Annual Review of Neuroscience·Lief E FennoKarl Deisseroth
Jul 13, 2011·Neuron·Ofer YizharKarl Deisseroth
Oct 22, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·I KahnC I Moore
Feb 22, 2012·Progress in Brain Research·Hongkui Zeng, Linda Madisen
Mar 8, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Seong-Gi Kim, Seiji Ogawa
Mar 13, 2012·Neuron·Christine Grienberger, Arthur Konnerth
May 9, 2012·Nature Methods·Kristina SchulzFritjof Helmchen
Jul 31, 2012·Current Biology : CB·Annelies GeritsWim Vanduffel
Feb 16, 2013·Biomedical Optics Express·Ioannis N PapadopoulosDemetri Psaltis
Jun 27, 2013·Nature Neuroscience·Adam M PackerMichael Häusser
Jul 23, 2013·Nature·Tsai-Wen ChenDouglas S Kim
Sep 6, 2013·Reports on Progress in Physics·Richard B Buxton
Nov 19, 2013·Nature Methods·Xin YuAlan P Koretsky
Feb 11, 2014·Nature Methods·Nathan C KlapoetkeEdward S Boyden
Mar 29, 2014·Science·Jonas WietekPeter Hegemann
Jul 7, 2014·Nature Neuroscience·Amy S ChuongEdward S Boyden

Related Concepts

In Vivo
Calcium [EPC]
Magnetic Resonance Imaging
Rod Opsins
Functional Neuroimaging

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.

Related Papers

Journal of Visualized Experiments : JoVE
Peter LinJin Hyung Lee
International Journal of Psychophysiology : Official Journal of the International Organization of Psychophysiology
Christoph S Herrmann, Stefan Debener
© 2020 Meta ULC. All rights reserved