Multimodal Microscopy Distinguishes Extracellular Aggregation and Cellular Uptake of Single-Walled Carbon Nanohorns

Chemistry : a European Journal
Stephen J DevereuxSusan J Quinn

Abstract

The low toxicity, high surface area, and ease of functionalisation of carbon nanohorns (CNH) makes them attractive systems for cellular imaging, diagnostics and therapeutics. However, challenges remain for the biomedical translation of these and other nanomaterials. A significant task is tuning the surface chemistry to achieve optimal cellular interactions. Herein, we combine real-time fluorescent imaging of nanoparticle cellular uptake and real-time differential interference contrast (DIC) imaging of extracellular media to monitor a) nanoparticle/nanoparticle and b) nanoparticle/cell interactions for CNHs covalently modified with an OFF/ON near-IR dye, the fluorescence of which is switched OFF in extracellular environments and triggered upon cellular internalisation. CHN samples modified with different loadings of the hydrophobic dye are taken as a simple model of drug-loaded nanoparticle systems. The punctate fluorescence suggests the CNHs are delivered to lysosomes and other vesicles of the endocytic pathway. DIC imaging highlights the competition that exists for many particle types, between extracellular aggregation and cellular internalization, the efficiency of which would be dependent upon the amount of fluorophore loadi...Continue Reading

References

May 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Jason D FeickDarrell Velegol
Dec 6, 2005·Molecular Pharmaceutics·Kumiko AjimaSumio Iijima
Apr 1, 2006·Chemical Communications : Chem Comm·John Killoran, Donal F O'Shea
Mar 3, 2007·Nature Materials·A K Geim, K S Novoselov
Mar 19, 2008·Journal of the American Chemical Society·Georgios RotasOsamu Ito
Sep 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Martin LundqvistKenneth A Dawson
Feb 12, 2009·ACS Nano·Jin MiyawakiSumio Iijima
Jun 16, 2009·Nature Materials·Andre E NelMike Thompson
Dec 18, 2009·Endocrinology·Banu S ZolnikMarina A Dobrovolskaia
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Scott E McNeil
Jun 22, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Xiaoxiao HeZhen Cheng
Oct 20, 2010·Nanoscale·Shuyun Zhu, Guobao Xu
Jan 20, 2011·Bioconjugate Chemistry·Frank ThielbeerMark Bradley
Feb 4, 2011·Angewandte Chemie·Wendelin J Stark
Nov 15, 2011·Journal of the American Chemical Society·Aniello PalmaDonal F O'Shea
Sep 26, 2012·The Journal of Organic Chemistry·Marco GrossiDonal F O'Shea
Jan 8, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Minfang ZhangMasako Yudasaka
Mar 14, 2013·Accounts of Chemical Research·Chul ChungDal-Hee Min
Apr 11, 2013·Expert Review of Molecular Diagnostics·Tae Hyung KimXiaoyuan Chen
Aug 28, 2013·The Journal of Physical Chemistry. a·L HawelekA Burian
Oct 8, 2014·Nature Reviews. Drug Discovery·Vladimir P Torchilin
Nov 20, 2014·Chemical Society Reviews·J BartelmessS Giordani
Jan 1, 2015·Beilstein Journal of Nanotechnology·Li ShangG Ulrich Nienhaus
Jan 20, 2015·Nanoscale·Minfang ZhangMasako Yudasaka
Jan 27, 2015·Annual Review of Physical Chemistry·Lucas A LaneShuming Nie
May 20, 2015·Chemical Society Reviews·Frederico R BaptistaS J Quinn
May 21, 2015·Chemical Communications : Chem Comm·Jia WenShiguo Sun
Sep 9, 2015·Nature Biotechnology·Elvin BlancoMauro Ferrari
Oct 2, 2015·Chemical Communications : Chem Comm·Dan WuDonal F O'Shea
Nov 19, 2015·Chemistry : a European Journal·Marco FrasconiSilvia Giordani
Apr 14, 2016·Chemical Reviews·Nikolaos KarousisNikos Tagmatarchis
May 18, 2016·Chemical Society Reviews·Yuan Ge, Donal F O'Shea
Oct 21, 2016·Bioconjugate Chemistry·Yuko NakamuraHisataka Kobayashi
Nov 12, 2016·Nature Reviews. Cancer·Jinjun ShiOmid C Farokhzad
May 2, 2017·European Journal of Medicinal Chemistry·Harrison C DalyDonal F O'Shea
Sep 19, 2017·Chemical Communications : Chem Comm·Dan Wu, Donal F O'Shea
Oct 28, 2017·Nature Reviews. Materials·Hongmin ChenXiaoyuan Chen

❮ Previous
Next ❯

Citations

Nov 17, 2020·Frontiers in Chemistry·Luis M MagnoSusan J Quinn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.