Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Young J KimOwen N Witte

Abstract

Previous work showed quantitative imaging of T-cell migration into a tumor site by positron emission tomography (PET), using retroviral transduction of mutated thymidine kinase (sr39TK) reporter genes into immunized T-lymphocytes. In order to improve the sensitivity and flexibility of the imaging analysis, lentivirus, that expressed sr39TK, was used to transduce the lymphocytes that migrated to an immunogenic sarcoma site. In comparison to retrovirally transduced lymphocytes, the lentivirally transduced lymphocytes showed enhanced PET signal when equal numbers of transduced lymphocytes were transferred. Furthermore, in order to utilize multimodality in vivo imaging capability, a tri-fusion reporter gene containing sr39TK, synthetic Renilla luciferase (hRluc), and enhanced green fluorescent protein (eGFP) was inserted into a lentiviral transfer vector. Using the adoptive transfer model, tumor-specific lymphocytic migration was detected by both microPET scan and bioluminescence imaging. The multimodal imaging strategy coupled with lentiviral reporter construct delivery demonstrated here can facilitate future molecular imaging studies.

References

Sep 1, 1991·Neurochemical Research·M E Phelps
Apr 1, 1990·Immunological Reviews·M PatarroyoC G Gahmberg
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·L NaldiniI M Verma
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S S GambhirH R Herschman
Sep 9, 1999·Radiology·R Weissleder
Oct 20, 1999·Methods : a Companion to Methods in Enzymology·A RosatoP Zanovello
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·S S GambhirH R Herschman
Feb 24, 2001·Histochemistry and Cell Biology·J WahlforsT Hakkarainen
Jun 15, 2001·ILAR Journal·S R Cherry, S S Gambhir
Oct 13, 2001·Trends in Molecular Medicine·P Price
Dec 4, 2001·Trends in Pharmacological Sciences·A J Reader, J Zweit
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·S Bhaumik, S S Gambhir
Feb 5, 2002·Immunity·Eric J Kunkel, Eugene C Butcher
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Alexander PfeiferInder M Verma
Feb 28, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Elisa VignaLuigi Naldini
Mar 21, 2002·Nature Reviews. Cancer·Ralph Weissleder
Sep 28, 2002·Journal of Magnetic Resonance Imaging : JMRI·Vijay SharmaDavid Piwnica-Worms
Oct 3, 2002·Annual Review of Pharmacology and Toxicology·Neeltje A Kootstra, Inder M Verma
Dec 3, 2002·Current Opinion in Biotechnology·Aaron C LoganDonald B Kohn
Jan 7, 2003·Nature Medicine·Ralph Weissleder, Vasilis Ntziachristos
Jan 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Purnima DubeyOwen N Witte
Jan 29, 2003·Journal of Cellular Biochemistry. Supplement·Stefanie MandlChristopher H Contag
Mar 12, 2003·Genes & Development·Tarik F Massoud, Sanjiv S Gambhir
Mar 26, 2003·Nature Biotechnology·Guenther KoehneJuri G Gelovani Tjuvajev
Apr 30, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Abhijit DeSanjiv Sam Gambhir
Jun 6, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiaobing YuLinzhao Cheng
Jun 14, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antoni RibasJames S Economou
Oct 25, 2003·Science·Harvey R Herschman
Feb 20, 2004·Cancer Research·Pritha RaySanjiv S Gambhir
Jan 4, 2008·Circulation·Moritz F KircherRalph Weissleder

❮ Previous
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Citations

Dec 6, 2005·European Journal of Nuclear Medicine and Molecular Imaging·Andreas H JacobsPedro Lowenstein
Feb 7, 2008·Cancer Immunology, Immunotherapy : CII·Robert M PrinsLinda M Liau
Mar 5, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Juan F VeraGianpietro Dotti
Sep 29, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Anna J Studwell, Darrell N Kotton
Feb 17, 2009·Nature Medicine·Elmer B SantosRenier J Brentjens
Feb 11, 2011·Molecular BioSystems·Gang LiuXiaoyuan Chen
Dec 17, 2010·Cancer Research·Manishkumar R PatelSanjiv S Gambhir
Jul 1, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Elizabeth J Akins, Purnima Dubey
Jun 17, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Konstantin DobrenkovVladimir Ponomarev
Nov 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Chengyi J ShuOwen N Witte
Apr 28, 2009·Expert Opinion on Biological Therapy·Wellington PhamJohn C Gore
May 9, 2014·BioMed Research International·Ya-Ju HsiehRen-Shyan Liu
Oct 27, 2015·Journal of Fluorescence·Jie DingGao-Jun Teng
Jul 28, 2006·Trends in Biotechnology·Giovanni LucignaniMario Clerici
Oct 26, 2005·Nuclear Medicine and Biology·Inna Serganova, Ronald Blasberg
Oct 11, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Luisa OttobriniGiovanni Lucignani
Jan 20, 2017·Science Translational Medicine·Khun Visith KeuSanjiv S Gambhir
May 17, 2006·Cell Transplantation·Philipp Mayer-KuckukJuri Gelovani
Jul 28, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mary E Dickinson
May 10, 2007·Nature Protocols·Shahriar S Yaghoubi, Sanjiv S Gambhir
Mar 9, 2017·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Vladimir Ponomarev
Aug 28, 2020·Cell Communication and Signaling : CCS·Dongfang LiuChen Liu
Sep 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Pritha RaySanjiv Sam Gambhir

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