Multinuclear magnetic resonance studies of the interaction of inorganic cations with heparin

Carbohydrate Research
D L RabensteinJ Peng

Abstract

The interaction of Na+, Ca2+, Mg2+, Zn2+ and La3+ with heparin, a highly negatively charged glycosaminoglycan, was studied by 1H and 23Na nuclear magnetic resonance spectroscopy. 1H chemical shift and nuclear Overhauser effect (NOE) data indicate that the counter ions Na+, Ca2+ and Mg2+ interact with the low pH, carboxylic acid form of heparin by delocalized, long-range electrostatic interactions. At higher pH, 1H chemical shift and NOE data indicate that Na+ and Mg2+ continue to interact with heparin in the same manner, even upon deprotonation of the carboxylic acid group; however, there is a site-specific contribution to the binding of Ca2+, Zn2+ and La3+ under these conditions. Acid dissociation constants for heparin carboxylic acid groups and heparin-metal binding constants were determined from the pH dependence of 1H chemical shifts and 23Na spin-lattice (T1) relaxation times. Equilibrium constants for exchange of M2+ for heparin-bound Na+ were obtained from 23Na T1 data. The acid dissociation constants show a strong dependence on Na+ concentration due to the polyelectrolyte character of heparin.

References

Mar 1, 1992·The Biochemical Journal·D GrantF B Williamson
Jul 15, 1992·The Biochemical Journal·D GrantF B Williamson
Jan 1, 1990·Annual Review of Biophysics and Biophysical Chemistry·C F Anderson, M T Record
Jan 1, 1989·Annals of the New York Academy of Sciences·E W Salzman
Jan 1, 1989·The Journal of Antimicrobial Chemotherapy·E De Clercq
Jan 1, 1989·Annals of the New York Academy of Sciences·J Hirsh
Jan 1, 1988·Archives of Biochemistry and Biophysics·G K HunterJ J Kim
Sep 1, 1980·The Biochemical Journal·G BengtssonU Lindahl
Sep 18, 1981·Biochimica Et Biophysica Acta·H GustavssonL A Fransson
Feb 1, 1981·The Biochemical Journal·R F Parrish, W R Fair
Jan 29, 1982·Biochemical and Biophysical Research Communications·W F Long, F B Williamson

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Citations

Aug 22, 2007·Pharmaceutical Research·Chengji Cui, Steven P Schwendeman
Jan 26, 2002·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shuzhen ZhangShenyang Tong
Feb 13, 2016·The Journal of Physical Chemistry. B·Khanh Nguyen, Dallas L Rabenstein
Aug 21, 2015·Journal of the Royal Society, Interface·Maria C Z MeneghettiMarcelo A Lima
Jan 22, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan OuJian-Ming Ouyang
Jul 21, 2016·Chemical Communications : Chem Comm·Severin J SiggCornelia G Palivan
Nov 8, 2005·Physical Chemistry Chemical Physics : PCCP·Lan JinDusan Uhrín
Apr 10, 2018·Journal of Alzheimer's Disease : JAD·Sergey P RadkoAlexander A Makarov
Nov 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Miloš Hricovíni, Michal Hricovíni
Sep 29, 2019·Medicina·Anthony DevlinMark Skidmore
Dec 7, 2007·The Journal of Physical Chemistry. a·Milan Remko, Claus-Wilhelm von der Lieth
Sep 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Ping JingShigeru Amemiya
May 1, 2021·Critical Reviews in Clinical Laboratory Sciences·G Lima-OliveiraP Ochoa
Sep 26, 2014·The Journal of Physical Chemistry. B·Miloš HricovíniOlga L Malkina
Sep 1, 2005·Analytical Chemistry·Jidong GuoShigeru Amemiya

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